Expression and localization of ecto-nucleotide pyrophosphatase/phosphodiesterase I-1 (E-NPP1/PC-1) and -3 (E-NPP3/CD203c/PD-Iβ/B10/gp130RB13-6) in inflammatory and neoplastic bile duct diseases

被引：27

作者：

Yano, Y

Hayashi, Y ^{[1
]}

Sano, K

Nagano, H

Nakaji, M

Seo, Y

Ninomiya, T

Yoon, S

Yokozaki, H

Kasuga, M

机构：

[1] Kobe Univ, Grad Sch Med, Div Mol Med & Med Genet, ICMR, Kobe, Hyogo 657, Japan

[2] Kobe Univ, Grad Sch Med, Dept Clin Mol Med, Div Diabet Digest & Kidney Dis, Kobe, Hyogo 657, Japan

[3] Kobe Univ, Grad Sch Med, Dept Dev & Geriatr Med, Div Pediat, Kobe, Hyogo 657, Japan

[4] Kobe Univ, Grad Sch Med, Dept Biomed Informat, Div Surg Pathol, Kobe, Hyogo 657, Japan

来源：

CANCER LETTERS | 2004年 / 207卷 / 02期

关键词：

E-NPP1; E-NPP3; bile duct carcinoma; primary biliary cirrhosis; carcinogenesis;

D O I：

10.1016/j.canlet.2003.11.002

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Ecto-nucleoticle phyrophosphatase/phosphodiesterase-1 enzyme (E-NPP) consists of three closely related molecules: E-NPP1, E-NPP2 and E-NPP3. We investigated the expression and localization of E-NPP1 and -3 in human inflammatory and neoplastic bile duct diseases. Immunohistochemically E-NPP I was located on the apical cytoplasmic side of cancer cells, whereas E-NPP3 was located in the apical plasma membrane. Western blot analysis revealed that the expression of E-NPP3, but not E-NPP1, was higher in tumor tissues than in surrounding tissues and the specific form of the E-NPP3 protein was readily detected in the sera of bile duct carcinoma (BDC) patients. Furthermore, it was confirmed that E-NPP3 was associated with migration ability by using of NIH3T3 cells that stably transfected with E-NPP3 cDNA. These results suggest that E-NPP3 is involved in the infiltration of neoplastic BDC and is possible to be a tumor marker. (C) 2003 Elsevier Ireland Ltd. All rights reserved.

引用

页码：139 / 147

页数：9

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