Upregulation of Lhx8 increase VAChT expression and ACh release in neuronal cell line SHSY5Y

被引：6

作者：

Li, Haoming ^{[1
]}

Jin, Guohua ^{[1
,2
]}

Zhu, Peipei ^{[3
]}

Zou, Linqing ^{[1
,2
]}

Shi, Jinhong ^{[1
,2
]}

Yi, Xin ^{[1
,2
]}

Zhang, Xinhua ^{[1
]}

Tian, Meiling ^{[1
]}

Qin, Jianbing ^{[1
]}

机构：

[1] Nantong Univ, Coll Med, Jiangsu Key Lab Neuroregenerat, Dept Human Anat, Nantong 226001, Jiangsu, Peoples R China

[2] Soochow Univ, Sch Med, Dept Human Anat & Histoembryol, Suzhou 215123, Peoples R China

[3] Shanghai Changzheng Hosp, Dept Pathol, Shanghai 200003, Peoples R China

来源：

NEUROSCIENCE LETTERS | 2014年 / 559卷

基金：

中国国家自然科学基金;

关键词：

Lhx8; ChAT; VAChT; ACh; SHSY5Y; VESICULAR ACETYLCHOLINE TRANSPORTER; LIM-HOMEOBOX GENE; CHOLINE-ACETYLTRANSFERASE; RETINOIC ACID; NEUROTROPHIC FACTOR; L3/LHX8; DIFFERENTIATION;

D O I：

10.1016/j.neulet.2013.11.047

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Lhx8 is a transcription factor for cholinergic differentiation. Our previous experiments found upregulation of Lhx8 promoted cholinergic neuronal differentiation of hippocampal neural stem/progenitor cells or hippocampal newborn neurons in vitro. However, the role of Lhx8 in VAChT expression and ACh release is still less understood. In this report, we transfected Lhx8 cDNA into neuronal cell line SHSY5Y by lentiviral vectors to acquire the cells which stably expressed high level of Lhx8. Using this cell model, we provided experimental evidence that increasing Lhx8 upregulated the expression of ChAT and VAChT, and also increased the ACh release in culture medium. We suggested that Lhx8 overexpression is a useful strategy to increase the release of ACh and maybe of therapeutic value to neurodegenerative diseases. (c) 2013 Elsevier Ireland Ltd. All rights reserved.

引用

页码：184 / 188

页数：5

共 21 条

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