Genome-wide identification of Chinese shrimp (Fenneropenaeus chinensis) microRNA responsive to low pH stress by deep sequencing

被引：14

作者：

He, Yuying ^{[1
,2
]}

Li, Zhaoxia ^{[3
]}

Zhang, Haien ^{[4
]}

Hu, Shuo ^{[4
]}

Wang, Qingyin ^{[1
]}

Li, Jian ^{[1
,2
]}

机构：

[1] Chinese Acad Fishery Sci, Key Lab Sustainable Dev Marine Fisheries, Minist Agr, Yellow Sea Fisheries Res Inst, Qingdao 266071, Shandong, Peoples R China

[2] Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266200, Shandong, Peoples R China

[3] Qingdao Agr Univ, Coll Marine Sci & Engn, Qingdao 266109, Shandong, Peoples R China

[4] Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China

来源：

CELL STRESS & CHAPERONES | 2019年 / 24卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Fenneropenaeus chinensis; microRNA; Low pH stress; HiSeq sequencing; PRAWN MACROBRACHIUM-ROSENBERGII; CARBONIC-ANHYDRASE GENES; LITOPENAEUS-VANNAMEI; ACID TOLERANCE; GROWTH; EXPOSURE; SURVIVAL; METABOLISM; PHYSIOLOGY; SALINITY;

D O I：

10.1007/s12192-019-00989-x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

pH has a great impact on the distribution, growth, behavior, and physiology in many aquatic animals. Here, we analyzed miRNA expression profiles of Chinese shrimp (Fenneropenaeus chinensis) from control pH (8.2) and low pH (6.5)-treated shrimp. Expression analysis identified 6 known miRNAs and 23 novel miRNAs with significantly different expression between control pH 8.2 and low pH 6.5; the predicted target genes of differentially expressed miRNAs were significantly enriched in organic acid metabolic process, oxidoreductase activity, coenzyme binding, cofactor binding, and collagen trimer. Moreover, target genes were significantly enriched in several Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways including citrate cycle, pyruvate metabolism, cytokine-cytokine receptor interaction, tight junction, carbon metabolism, etc. Our survey expanded the number of known shrimp miRNAs and provided comprehensive information about miRNA response to low pH stress.

引用

页码：689 / 695

页数：7

共 32 条

[1] Expression patterns of two carbonic anhydrase genes, Na+/K+-ATPase and V-type H+-ATPase, in the freshwater crayfish, Cherax quadricarinatus, exposed to low pH and high pH [J].