Molecular cloning and characterization of a venom phospholipase A2 from the bumblebee Bombus ignitus

被引：36

作者：

Xin, Yu ^{[1
,2
,3
]}

Choo, Young Moo ^{[1
]}

Hu, Zhigang ^{[1
]}

Lee, Kwang Sik ^{[1
]}

Yoon, Hyung Joo ^{[4
]}

Cui, Zheng ^{[2
,3
]}

Sohn, Hung Dae ^{[1
]}

Jin, Byung Rae ^{[1
,2
,3
]}

机构：

[1] Dong A Univ, Coll Nat Resources & Life Sci, Pusan 604714, South Korea

[2] Dong A Univ, Joint Lab, Shenyang, Peoples R China

[3] Shenyang Pharmaceut Univ, Shenyang, Peoples R China

[4] Natl Acad Agr Sci, Dept Agr Biol, Suwon 441100, South Korea

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 2009年 / 154卷 / 02期

关键词：

Bombus ignitus; Bumblebee; Enzyme; Insect; Phospholipase A(2); Venom; HONEYBEE; ALLERGY; TEMPERATURE; DIVERSITY;

D O I：

10.1016/j.cbpb.2009.06.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phospholipase A(2) (PLA(2)) is one of the main components of bee venom. Here, we identify a venom PLA(2) from the bumblebee, Bombus ignitus. Bumblebee venom PLA(2) (Bi-PLA(2)) cDNA, which was identified by searching B. ignitus venom gland expressed sequence tags, encodes a 180 amino acid protein. Comparison of the genomic sequence with the cDNA sequence revealed the presence of four exons and three introns in the Bi-PLA(2) gene. Bi-PLA(2) is an 18-kDa glycoprotein. It is expressed in the venom gland, cleaved between the residues Arg44 and Ile45, and then stored in the venom sac. Comparative analysis revealed that the mature Bi-PLA(2) (136 amino acids) possesses features consistent with other bee PLA(2)S, including ten conserved cysteine residues, as well as a highly conserved Ca2+-binding site and active site. Phylogenetic analysis of bee PLA(2)s separated the bumblebee and honeybee PLA(2) proteins into two groups. The mature Bi-PLA(2) purified from the venom of B. ignitus worker bees hydrolyzed DBPC, a known substrate of PLA(2). Immunofluorescence staining of Bi-PLA(2)-treated insect Sf9 cells revealed that Bi-PLA(2) binds at the cell membrane and induces apoptotic cell death. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：195 / 202

页数：8

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