The comet assay as a method of assessment of neurotoxicity - Usefulness for drugs of abuse

被引：12
作者：
Frenzilli, Giada
Scarcelli, Vittoria
Fornai, Francesco
Paparelli, Antonio
Nigro, Marco
机构：
[1] Univ Pisa, Dipartimento Morfol Umana & Biol Applicata, I-56126 Pisa, Italy
[2] INM Neuromed, IRCCS, Lab Neurobiol Movement Disorders, I-86077 Isernia, Italy
来源：
CELLULAR AND MOLECULAR MECHANISMS OF DRUGS OF ABUSE AND NEUROTOXICITY: COCAINE, GHB, AND SUBSTITUTED AMPHETAMINES | 2006年 / 1074卷
关键词：
DNA damage; neurotoxicity; Comet assay; drugs of abuse; brain cells;
D O I：
10.1196/annals.1369.048
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Comet assay is a quick and versatile method for assessing DNA damage in individual cells. It allows the detection of single and double DNA strand breaks, as well as the presence of alkali labile sites. DNA breaks may represent the direct effect of some damaging agent, or they may be intermediates in cellular repair. DNA strand breaks may also come from the action of free radicals generated by oxidative stress processes. The present article summarizes some data from our and other groups underlining the suitability of the Comet assay in assessing neurotoxicity and its potential in evaluating drugs of abuse-related genotoxicity.
引用
收藏
页码：478 / 481
页数：4
相关论文
共 23 条
[1]   Tiagabine treatment and DNA damage in rat astrocytes: an in vitro study by comet assay [J].
Cardile, V ;
Palumbo, M ;
Renis, M ;
Pavone, A ;
Maci, T ;
Perciavalle, V .
NEUROSCIENCE LETTERS, 2001, 306 (1-2) :17-20
[2]   Differential effect of cycloheximide on neuronal and glioma cells treated with chemotherapy and radiation [J].
Chao, KSC ;
Hsu, JSJ ;
Xu, J ;
Ezekiel, UR ;
Eves, E ;
Rosner, M ;
Hsu, CY .
JOURNAL OF NEURO-ONCOLOGY, 1999, 45 (01) :19-26
[3]   The comet assay for DNA damage and repair - Principles, applications, and limitations [J].
Collins, AR .
MOLECULAR BIOTECHNOLOGY, 2004, 26 (03) :249-261
[4]   DNA damage and ubiquitinated neuronal inclusions in the substantia nigra and striatum of mice following MDMA (ecstasy) [J].
Fornai, F ;
Lenzi, P ;
Frenzilli, G ;
Gesi, M ;
Ferrucci, M ;
Lazzeri, G ;
Biagioni, F ;
Nigro, M ;
Falleni, A ;
Giusiani, M ;
Pellegrini, A ;
Blandini, F ;
Ruggieri, S ;
Paparelli, A .
PSYCHOPHARMACOLOGY, 2004, 173 (3-4) :353-363
[5]   Acute exposure of cultured neurones to ethanol results in reversible DNA single-strand breaks; Whereas chronic exposure causes loss of cell viability [J].
Lamarche, F ;
Gonthier, B ;
Signorini, N ;
Eysseric, H ;
Barret, L .
ALCOHOL AND ALCOHOLISM, 2003, 38 (06) :550-558
[6]   Measurement of DNA damage alter exposure to 2450 MHz electromagnetic radiation [J].
Malyapa, RS ;
Ahern, EW ;
Straube, WL ;
Moros, EG ;
Pickard, WF ;
Roti, JLR .
RADIATION RESEARCH, 1997, 148 (06) :608-617
[7]   DNA damage and DNA adduct formation in rat tissues following oral administration of acrylamide [J].
Manière, I ;
Godard, T ;
Doerge, DR ;
Churchwell, MI ;
Guffroy, M ;
Laurentie, M ;
Poul, JM .
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2005, 580 (1-2) :119-129
[8]   DNA damage and apoptosis in the immature mouse cerebellum after acute exposure to a 1 mT, 60 Hz magnetic field [J].
McNamee, JP ;
Bellier, PV ;
McLean, JRN ;
Marro, L ;
Gajda, GB ;
Thansandote, A .
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2002, 513 (1-2) :121-133
[9]   Oxidative DNA damage in peripheral leukocytes of mild cognitive impairment and AD patients [J].
Mighore, L ;
Fontana, I ;
Trippi, F ;
Colognato, R ;
Coppedè, F ;
Tognoni, G ;
Nucciarone, B ;
Siciliano, G .
NEUROBIOLOGY OF AGING, 2005, 26 (05) :567-573
[10]   Oxidative damage and cytogenetic analysis in leukocytes of Parkinson's disease patients [J].
Migliore, L ;
Petrozzi, L ;
Lucetti, C ;
Gambaccini, G ;
Bernardini, S ;
Scarpato, R ;
Trippi, F ;
Barale, R ;
Frenzilli, G ;
Rodilla, V ;
Bonuccelli, U .
NEUROLOGY, 2002, 58 (12) :1809-1815
123