Rapid-scan EPR of immobilized nitroxides

被引：18

作者：

Yu, Zhelin ^{[1
]}

Quine, Richard W. ^{[2
]}

Rinard, George A. ^{[2
]}

Tseitlin, Mark ^{[1
]}

Elajaili, Hanan ^{[1
]}

Kathirvelu, Velavan ^{[1
]}

Clouston, Laura J. ^{[3
]}

Boratynski, Przemyslaw J. ^{[3
]}

Rajca, Andrzej ^{[3
]}

Stein, Richard ^{[4
]}

Mchaourab, Hassane ^{[4
]}

Eaton, Sandra S. ^{[1
]}

Eaton, Gareth R. ^{[1
]}

机构：

[1] Univ Denver, Dept Chem & Biochem, Denver, CO 80208 USA

[2] Univ Denver, Sch Engn & Comp Sci, Denver, CO 80208 USA

[3] Univ Nebraska, Dept Chem, Lincoln, NE 68588 USA

[4] Vanderbilt Univ, Med Ctr, Dept Mol Physiol & Biophys, Nashville, TN 37232 USA

来源：

JOURNAL OF MAGNETIC RESONANCE | 2014年 / 247卷

关键词：

Immobilized nitroxide; Litz wire coils; Rapid scan EPR; Sucrose octaacetate glass; Trehalose glass; ELECTRON-SPIN RELAXATION; LATTICE-RELAXATION; CONTINUOUS-WAVE; RADICALS; SPECTROSCOPY; ABSORPTION; LIQUIDS; RATES; TIMES; ECHO;

D O I：

10.1016/j.jmr.2014.08.008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

X-band electron paramagnetic resonance spectra of immobilized nitroxides were obtained by rapid scan at 293 K. Scan widths were 155 G with 13.4 kHz scan frequency for N-14-perdeuterated tempone and for T4 lysozyme doubly spin labeled with an iodoacetamide spirocyclohexyl nitroxide and 100 G with 20.9 kHz scan frequency for N-15-perdeuterated tempone. These wide scans were made possible by modifications to our rapid-scan driver, scan coils made of Litz wire, and the placement of highly conducting aluminum plates on the poles of a Bruker 10 '' magnet to reduce resistive losses in the magnet pole faces. For the same data acquisition time, the signal-to-noise for the rapid-scan absorption spectra was about an order of magnitude higher than for continuous wave first-derivative spectra recorded with modulation amplitudes that do not broaden the lineshapes. (C) 2014 Elsevier Inc. All rights reserved.

引用

页码：67 / 71

页数：5

共 26 条

[1] Electron spin-lattice relaxation mechanisms of rapidly-tumbling nitroxide radicals [J].

Biller, Joshua R. ;

Elajaili, Hanan ;

Meyer, Virginia ;

Rosen, Gerald M. ;

Eaton, Sandra S. ;

Eaton, Gareth R. .

JOURNAL OF MAGNETIC RESONANCE, 2013, 236 :47-56

[2] Nonlinear-least-squares analysis of slow-motion EPR spectra in one and two dimensions using a modified Levenberg-Marquardt algorithm [J].

Budil, DE ;

Lee, S ;

Saxena, S ;

Freed, JH .

JOURNAL OF MAGNETIC RESONANCE SERIES A, 1996, 120 (02) :155-189

[3]

Eaton SS, 2014, MULTIFREQUENCY ELECTRON PARAMAGNETIC RESONANCE: DATA AND TECHNIQUES, P3

[4]

Eaton SS, 2000, BIO MAGN RE, V19, P29

[5]

Freed J.H., 1976, SPIN LABELING, P53

[6] Moving difference (MDIFF) non-adiabatic rapid sweep (NARS) EPR of copper(II) [J].

Hyde, James S. ;

Bennett, Brian ;

Kittell, Aaron W. ;

Kowalski, Jason M. ;

Sidabras, Jason W. .

JOURNAL OF MAGNETIC RESONANCE, 2013, 236 :15-25

[7] Relaxation rates for spirocyclohexyl nitroxyl radicals are suitable for interspin distance measurements at temperatures up to about 125 K [J].

Kathirvelu, Velavan ;

Smith, Christopher ;

Parks, Christopher ;

Mannan, Md. Abdul ;

Miura, Yozo ;

Takeshita, Keizo ;

Eaton, Sandra S. ;

Eaton, Gareth R. .

CHEMICAL COMMUNICATIONS, 2009, (04) :454-456

[8]

Meyer V., 2014, UNPUB

[9] TWO-DIMENSIONAL ELECTRON-SPIN ECHO SPECTROSCOPY AND SLOW MOTIONS [J].

MILLHAUSER, GL ;

FREED, JH .

JOURNAL OF CHEMICAL PHYSICS, 1984, 81 (01) :37-48

[10] X-band rapid-scan EPR of samples with long electron spin relaxation times: a comparison of continuous wave, pulse and rapid-scan EPR [J].

Mitchell, Deborah G. ;

Tseitlin, Mark ;

Quine, Richard W. ;

Meyer, Virginia ;

Newton, Mark E. ;

Schnegg, Alexander ;

George, Benjamin ;

Eaton, Sandra S. ;

Eaton, Gareth R. .

MOLECULAR PHYSICS, 2013, 111 (18-19) :2664-2673

← 1 2 3 →