Enhanced DNA repair by DNA photolyase bearing an artificial light-harvesting chromophore

被引:8
作者
Terai, Yuma [1 ]
Sato, Ryuma [2 ]
Matsumura, Risa [1 ]
Iwai, Shigenori [1 ]
Yamamoto, Junpei [1 ]
机构
[1] Osaka Univ, Grad Sch Engn Sci, Div Chem, 1-3 Machikaneyama, Toyonaka, Osaka 5608531, Japan
[2] RIKEN, Ctr Biosyst Dynam Res, 6-2-3 Furuedai, Suita, Osaka 5650874, Japan
关键词
ESCHERICHIA-COLI; ENERGY-TRANSFER; ANTENNA CHROMOPHORE; CRYSTAL-STRUCTURE; MOLECULAR-DYNAMICS; ELECTRON-TRANSFER; BINDING; PROTEIN; IDENTIFICATION; SUBSTRATE;
D O I
10.1093/nar/gkaa719
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Photolyases are flavoenzymes responsible for the repair of carcinogenic DNA damage caused by ultraviolet radiation. They harbor the catalytic cofactor flavin adenine dinucleotide (FAD). The light-driven electron transfer from the excited state of the fully-reduced form of FAD to the DNA lesions causes rearrangement of the covalent bonds, leading to the restoration of intact nucleobases. In addition to the catalytic chromophore, some photolyases bear a secondary chromophore with better light absorption capability than FAD, acting as a light-harvesting chromophore that harvests photons in sunlight efficiently and transfers light energy to the catalytic center, as observed in natural photoreceptor proteins. Inspired by nature, we covalently and site-specifically attached a synthetic chromophore to the surface of photolyase using oligonucleotides containing a modified nucleoside and a cyclobutane-type DNA lesion, and successfully enhanced its enzymatic activity in the light-driven DNA repair. Peptide mapping in combination with theoretical calculations identified the amino acid residue that binds to the chromophore, working as an artificial light-harvesting chromophore. Our results broaden the strategies for protein engineering and provide a guideline for tuning of the light perception abilities and enzymatic activity of the photoreceptor proteins.
引用
收藏
页码:10076 / 10086
页数:11
相关论文
共 50 条
[41]   Natural and artificial light-harvesting systems utilizing the functions of carotenoids [J].
Hashimoto, Hideki ;
Sugai, Yuko ;
Uragami, Chiasa ;
Gardiner, Alastair T. ;
Cogdell, Richard J. .
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY C-PHOTOCHEMISTRY REVIEWS, 2015, 25 :46-70
[42]   An Artificial Light-Harvesting System based on Supramolecular AIEgen Assembly [J].
Jia, Dan ;
Luo, Quan ;
Liu, Shicong ;
Hou, Chunxi ;
Liu, Junqiu .
CHEMISTRY-A EUROPEAN JOURNAL, 2024, 30 (54)
[43]   The coherence time of sunlight in the context of natural and artificial light-harvesting [J].
Ricketti, Berke Vow ;
Gauger, Erik M. ;
Fedrizzi, Alessandro .
SCIENTIFIC REPORTS, 2022, 12 (01)
[44]   Enhanced thermal stability enables human mismatch-specific thymine-DNA glycosylase to catalyse futile DNA repair [J].
Manapkyzy, Diana ;
Joldybayeva, Botagoz ;
Ishchenko, Alexander A. ;
Matkarimov, Bakhyt T. ;
Zharkov, Dmitry O. ;
Taipakova, Sabira ;
Saparbaev, Murat K. .
PLOS ONE, 2024, 19 (10)
[45]   Dynamic artificial light-harvesting systems based on rotaxane dendrimers [J].
Li, Wei-Jian ;
Wang, Xu-Qing ;
Wang, Wei ;
Hu, Zhubin ;
Ke, Yubin ;
Jiang, Hanqiu ;
He, Chunyong ;
Wang, Xueli ;
Hu, Yi-Xiong ;
Jia, Pei-Pei ;
Yin, Panchao ;
Chen, Jinquan ;
Sun, Haitao ;
Sun, Zhenrong ;
Xu, Lin ;
Yang, Hai-Bo .
GIANT, 2020, 2
[46]   Artificial light-harvesting antenna systems grafted on a carbohydrate platform [J].
Bonaccorsi, Paola ;
Aversa, Maria Chiara ;
Barattucci, Anna ;
Papalia, Teresa ;
Puntoriero, Fausto ;
Campagna, Sebastiano .
CHEMICAL COMMUNICATIONS, 2012, 48 (85) :10550-10552
[47]   A Supramolecular Artificial Light-Harvesting System with an Ultrahigh Antenna Effect [J].
Li, Jing-Jing ;
Chen, Yong ;
Yu, Jie ;
Cheng, Ni ;
Liu, Yu .
ADVANCED MATERIALS, 2017, 29 (30)
[48]   Discrete Cyclic Porphyrin Arrays as Artificial Light-Harvesting Antenna [J].
Aratani, Naoki ;
Kim, Dongho ;
Osuka, Atsuhiro .
ACCOUNTS OF CHEMICAL RESEARCH, 2009, 42 (12) :1922-1934
[49]   Electron Tunneling Pathways and Role of Adenine in Repair of Cyclobutane Pyrimidine Dimer by DNA Photolyase [J].
Liu, Zheyun ;
Guo, Xunmin ;
Tan, Chuang ;
Li, Jiang ;
Kao, Ya-Ting ;
Wang, Lijuan ;
Sancar, Aziz ;
Zhong, Dongping .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2012, 134 (19) :8104-8114
[50]   Enhanced Photocatalysis of Rhenium(I) Complex by Light-Harvesting Periodic Mesoporous Organosilica [J].
Takeda, Hiroyuki ;
Ohashi, Masataka ;
Tani, Takao ;
Ishitani, Osamu ;
Inagaki, Shinji .
INORGANIC CHEMISTRY, 2010, 49 (10) :4554-4559