Regulatory T Cells and Extracorporeal Photochemotherapy: Correlation With Clinical Response and Decreased Frequency of Proinflammatory T Cells

被引：61

作者：

Di Biaso, Iolanda ^{[1
]}

Di Maio, Lucia ^{[1
]}

Bugarin, Cristina ^{[1
]}

Gaipa, Giuseppe ^{[1
]}

Dander, Erica ^{[1
]}

Balduzzi, Adriana ^{[1
]}

Parma, Matteo ^{[2
]}

D'Amico, Giovanna ^{[1
]}

Perseghin, Paolo ^{[3
]}

Biondi, Andrea ^{[1
]}

Biagi, Ettore

机构：

[1] Univ Milano Bicocca, San Gerardo Hosp, Dept Pediat, Lab Cell Therapy Stefano Verri, I-20052 Milan, Italy

[2] San Gerardo Hosp, Bone Marrow Transplantat Unit, Dept Adult Hematol, Milan, Italy

[3] San Gerardo Hosp, Dept Clin Pathol, Immunohematol & Transfus Therapeut Aphaeresis Uni, Milan, Italy

来源：

TRANSPLANTATION | 2009年 / 87卷 / 09期

关键词：

Extracorporeal photochemotherapy; Regulatory T cells; GvHD; Th-17; VERSUS-HOST-DISEASE; MECHANISMS; EXPRESSION;

D O I：

10.1097/TP.0b013e3181a27a5d

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Background. Immune mechanisms of extracorporeal photochernotherapy (ECP) in refractory/resistant graft-versus-host disease (GvHD) are complex. We have previously analyzed the role of CD4(+)CD25(+)Foxp3(+) regulatory T cells (T-regs). Methods. In the current study, we have enlarged the size of the population (n=27; chronic GvHD=18, acute GvHD=9) for a median follow-up of 24 months. T-regs were monitored for CD4, CD25, glucocorticoid-induced tumor necrosis factor receptor (GITR), CD62L, CCR7, Foxp3, and STAT-5. Immune analysis by interleukin (IL)-17 Elispot was carried out on circulating T-helper CD4(+) cells secreting IL-17, a subset of T cells considered relevant in the pathogenesis of GvHD. Results. We confirm that ECP is accompanied by a significant increase of CD4(+)CD25(+)Foxp3(+)GITR(+)CD62L(+)CCR7(+) T-regs. Sorted T-regs show augmented phosphorylation of STAT-5. Only ECP-responding patients demonstrate a raise of circulating T-regs, being mostly affected by chronic GvHD. Moreover, this phenomenon corresponds to a diminished secretion of IL-17. Discussion. In conclusion, our study shows that T-regs represent important immune mediators of the clinical benefits of ECP in patients affected by GvHD.

引用

页码：1422 / 1425

页数：4

共 10 条

[1] Extracorporeal photochemotherapy is accompanied by increasing levels of circulating CD4+CD25+GITR+Foxp3+CD62L+functional regulatory T-cells in patients with graft-versus-host disease [J].