Regulatory T Cells and Extracorporeal Photochemotherapy: Correlation With Clinical Response and Decreased Frequency of Proinflammatory T Cells

被引:61
作者
Di Biaso, Iolanda [1 ]
Di Maio, Lucia [1 ]
Bugarin, Cristina [1 ]
Gaipa, Giuseppe [1 ]
Dander, Erica [1 ]
Balduzzi, Adriana [1 ]
Parma, Matteo [2 ]
D'Amico, Giovanna [1 ]
Perseghin, Paolo [3 ]
Biondi, Andrea [1 ]
Biagi, Ettore
机构
[1] Univ Milano Bicocca, San Gerardo Hosp, Dept Pediat, Lab Cell Therapy Stefano Verri, I-20052 Milan, Italy
[2] San Gerardo Hosp, Bone Marrow Transplantat Unit, Dept Adult Hematol, Milan, Italy
[3] San Gerardo Hosp, Dept Clin Pathol, Immunohematol & Transfus Therapeut Aphaeresis Uni, Milan, Italy
关键词
Extracorporeal photochemotherapy; Regulatory T cells; GvHD; Th-17; VERSUS-HOST-DISEASE; MECHANISMS; EXPRESSION;
D O I
10.1097/TP.0b013e3181a27a5d
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Background. Immune mechanisms of extracorporeal photochernotherapy (ECP) in refractory/resistant graft-versus-host disease (GvHD) are complex. We have previously analyzed the role of CD4(+)CD25(+)Foxp3(+) regulatory T cells (T-regs). Methods. In the current study, we have enlarged the size of the population (n=27; chronic GvHD=18, acute GvHD=9) for a median follow-up of 24 months. T-regs were monitored for CD4, CD25, glucocorticoid-induced tumor necrosis factor receptor (GITR), CD62L, CCR7, Foxp3, and STAT-5. Immune analysis by interleukin (IL)-17 Elispot was carried out on circulating T-helper CD4(+) cells secreting IL-17, a subset of T cells considered relevant in the pathogenesis of GvHD. Results. We confirm that ECP is accompanied by a significant increase of CD4(+)CD25(+)Foxp3(+)GITR(+)CD62L(+)CCR7(+) T-regs. Sorted T-regs show augmented phosphorylation of STAT-5. Only ECP-responding patients demonstrate a raise of circulating T-regs, being mostly affected by chronic GvHD. Moreover, this phenomenon corresponds to a diminished secretion of IL-17. Discussion. In conclusion, our study shows that T-regs represent important immune mediators of the clinical benefits of ECP in patients affected by GvHD.
引用
收藏
页码:1422 / 1425
页数:4
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