Role of Fatty Acid Synthase in the Development of Dictyostelium discoideum

被引:1
作者
Narita, Takaaki B. [1 ]
Kikukawa, Takahiro W. [1 ]
Sato, Yukie G. [1 ]
Miyazaki, Saki H. [1 ]
Morita, Naoki [2 ]
Saito, Tamao [3 ]
机构
[1] Sophia Univ, Grad Sch Sci & Technol, Tokyo 1028554, Japan
[2] Natl Inst Adv Ind Sci & Technol, Bioprod Res Inst, Sapporo, Hokkaido 0628517, Japan
[3] Sophia Univ, Dept Mat & Life Sci, Tokyo 1028554, Japan
基金
日本学术振兴会;
关键词
fatty acid synthase; development; knockout mutant; Dictyostelium; DIFFERENTIATION-INDUCING FACTOR; IDENTIFICATION; DESATURASE; CERULENIN;
D O I
10.5650/jos.ess13142
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Fatty acids are fundamental cellular components, and provide essential building blocks for membrane biosynthesis. Although the use of gene knockout mutants is a robust method for examining the function of specific cellular metabolic networks, fatty acid synthase knockout mutants are extremely difficult to isolate. In the Dictyostelium discoideum genome, we found two putative fatty acid synthase genes, and we created a knockout mutant for one of them to examine the physiological consequences. In this study, we found that a continuous fatty acid supply was necessary for normal development, and the fatty acid synthase knockout mutant showed severe developmental delay. This developmental defect was corrected in chimeras composed of wild type cells and knockout mutant cells (3:7, respectively). The knockout mutant also showed aberrant expression of fatty acid biosynthesis genes. These results showed that D. discoideum needs correct fatty acid synthesis for normal development.
引用
收藏
页码:281 / 289
页数:9
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