The Making of a Macromolecular Machine: Assembly of the Membrane Attack Complex

被引：51

作者：

Bubeck, Doryen ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Dept Life Sci, London SW7 2AZ, England

来源：

BIOCHEMISTRY | 2014年 / 53卷 / 12期

关键词：

PORE FORMATION; BINDING-SITE; HUMAN CD59; PERFRINGOLYSIN-O; STRUCTURAL BASIS; CRYSTAL-STRUCTURE; PROVIDE INSIGHTS; PROTEIN C8-ALPHA; 8TH COMPONENT; MACPF DOMAIN;

D O I：

10.1021/bi500157z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The complement terminal pathway clears pathogens by generating cytotoxic membrane attack complex (MAC) pores on target cells. For more than 40 years, biochemical and cellular assays have been used to characterize the lytic nature of the MAC and to define its protein composition. Although models for pore formation have been inferred from structures of bacterial cytolysins, it was only recently that we were able to visualize how complement components come together during MAC assembly. This review highlights structural analyses of terminal pathway complexes to explore molecular mechanisms underlying MAC formation.

引用

页码：1908 / 1915

页数：8

共 82 条

[71] Identification of a membrane-spanning domain of the thiol-activated pore-forming toxin Clostridium perfringens perfringolysin O:: An α-helical to β-sheet transition identified by fluorescence spectroscopy [J].

Shepard, LA ;

Heuck, AP ;

Hamman, BD ;

Rossjohn, J ;

Parker, MW ;

Ryan, KR ;

Johnson, AE ;

Tweten, RK .

BIOCHEMISTRY, 1998, 37 (41) :14563-14574

[72] Crystal structure of the MACPF domain of human complement protein C8α in complex with the C8γ subunit [J].

Slade, Daniel J. ;

Lovelace, Leslie L. ;

Chruszcz, Maksymilian ;

Minor, Wladek ;

Lebioda, Lukasz ;

Sodetz, James M. .

JOURNAL OF MOLECULAR BIOLOGY, 2008, 379 (02) :331-342

[73] Functional studies of the MACPF domain of human complement protein C8α reveal sites for simultaneous binding of C8β, C8γ, and C9 [J].

Slade, DJ ;

Chiswell, B ;

Sodetz, JM .

BIOCHEMISTRY, 2006, 45 (16) :5290-5296

[74] Specific protein-membrane contacts are required for prepore and pore assembly by a cholesterol-dependent cytolysin [J].

Soltani, Casie E. ;

Hotze, Eileen M. ;

Johnson, Arthur E. ;

Tweten, Rodney K. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2007, 282 (21) :15709-15716

[75]

STECKEL EW, 1983, J BIOL CHEM, V258, P4318

[76] ANALYSIS OF THE SPECIFIC ASSOCIATION OF THE 8TH AND 9TH COMPONENTS OF HUMAN-COMPLEMENT - IDENTIFICATION OF A DIRECT ROLE FOR THE ALPHA-SUBUNIT OF C8 [J].

STEWART, JL ;

SODETZ, JM .

BIOCHEMISTRY, 1985, 24 (17) :4598-4602

[77] Structural basis of pore formation by the bacterial toxin pneumolysin [J].

Tilley, SJ ;

Orlova, EV ;

Gilbert, RJC ;

Andrew, PW ;

Saibil, HR .

CELL, 2005, 121 (02) :247-256

[78] ULTRASTRUCTURE OF THE MEMBRANE ATTACK COMPLEX OF COMPLEMENT - DETECTION OF THE TETRAMOLECULAR C9-POLYMERIZING COMPLEX C5B-8 [J].

TSCHOPP, J ;

PODACK, ER ;

MULLEREBERHARD, HJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (23) :7474-7478

[79]

TSCHOPP J, 1984, J BIOL CHEM, V259, P7857

[80] Membrane pore formation by human complement: Functional importance of the transmembrane β-hairpin (TMH) segments of C80α and C9 [J].

Weiland, Mitch H. ;

Qian, Yu ;

Sodetz, James M. .

MOLECULAR IMMUNOLOGY, 2014, 57 (02) :310-316

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