JNK MAP kinase is involved in the humoral immune response of the greater wax moth larvae Galleria mellonella

被引:30
作者
Wojda, I [1 ]
Kowalski, P [1 ]
Jakubowicz, T [1 ]
机构
[1] Marie Curie Sklodowska Univ, Dept Invertebrate Immunol, Inst Biol, PL-20033 Lublin, Poland
关键词
Galleria mellonella; insect immunity; JNK MAP kinase; protein phosphorylation;
D O I
10.1002/arch.20001
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We investigated the participation of MAP kinases in the response of Galleria mellonella larvae to immune challenge. JNK MAP kinase was activated in fat body 10-15 min after LPS injection in vivo. The level of JNK activation was time- and LPS dosage-dependent. INK MAP kinase isolated from cell-free extract of fat bodies dissected from immune stimulated larvae phosphorylated c-Jun protein in vitro. The activity of Gm JNK kinase was abolished in the presence of the JNK specific inhibitor SP600125. Our data indicate a correlation between JNK phosphorylation and induction of antimicrobial activity in the insect hemolymph after immune stimulation. Hemolymph from larvae pre-treated with JNK specific inhibitor SP600125 showed a reduced level of antibacterial activity after LPS injection. JNK inhibition by SP600125 abolished antibacterial activity of the in vitro culture of G. mellonella fat body. Finally, we also show a correlation between JNK-dependent immune response of G. mellonella larvae and elevated temperature. Arch. Insect Biochem. (C) 2004 Wiley-Liss, Inc.
引用
收藏
页码:143 / 154
页数:12
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