Simultaneous determination of residual fourteen kinds of β-lactam and macrolide antibiotics in bovine muscles by high-performance liquid chromatography with a diode array detector

被引：5

作者：

Nagata, T ^{[1
]}

Ashizawa, E ^{[1
]}

Hashimoto, H ^{[1
]}

机构：

[1] Chiba Prefectural Inst Publ Hlth, Chuo Ku, Chiba 2608715, Japan

来源：

JOURNAL OF THE FOOD HYGIENIC SOCIETY OF JAPAN | 2004年 / 45卷 / 03期

关键词：

veterinary drug; beta-lactam antibiotic; macrolide antibiotic; bovine muscle; HPLC;

D O I：

10.3358/shokueishi.45.161

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Residues of 14 kinds of beta-lactam and macrolide antibiotics in bovine muscles were extracted with acetonitrile and the extract was partitioned with n-hexane, to remove fat. High-performance liquid chromatography was carried out on a TSK-gel ODS-80TM column using gradient elution with acetonitrile-0.05% trifluoroacetic acid and the drugs were quantitated by diode array detection. The recoveries of the drugs from bovine muscles spiked at 0.1 ppm were over 63% and each quantitation limit was 0.04 ppm.

引用

页码：161 / 164

页数：4

共 9 条

[1] CONFIRMATORY ASSAY FOR THE SIMULTANEOUS DETECTION OF 5 PENICILLINS IN MUSCLE, KIDNEY AND MILK USING LIQUID-CHROMATOGRAPHY - ELECTROSPRAY MASS-SPECTROMETRY [J].

BLANCHFLOWER, WJ ;

HEWITT, SA ;

KENNEDY, DG .

ANALYST, 1994, 119 (12) :2595-2601

[2]

Delepine B, 1996, J AOAC INT, V79, P397

[3] Simultaneous determination of five penicillins in muscle, liver and kidney from slaughtered animals using liquid chromatography coupled with electrospray ionization tandem mass spectrometry [J].

Hatano, K .

JOURNAL OF THE FOOD HYGIENIC SOCIETY OF JAPAN, 2003, 44 (01) :1-6

[4] Simultaneous determination of five macrolide antibiotics in meat by high-performance liquid chromatography [J].

Horie, M ;

Saito, K ;

Ishii, R ;

Yoshida, T ;

Haramaki, Y ;

Nakazawa, H .

JOURNAL OF CHROMATOGRAPHY A, 1998, 812 (1-2) :295-302

[5] Application of ion-exchange cartridge clean-up in food analysis III. Determination of benzylpenicillin, phenoxymethyl-penicillin, oxacillin, cloxacillin, nafcillin and dicloxacillin in bovine liver and kidney by liquid chromatography with ultraviolet detection [J].

Ito, Y ;

Ikai, Y ;

Oka, H ;

Matsumoto, H ;

Kagami, T ;

Takeba, K .

JOURNAL OF CHROMATOGRAPHY A, 2000, 880 (1-2) :85-91

[6] Application of ion-exchange cartridge clean-up in food analysis IV. Confirmatory assay of benzylpenicillin, phenoxymethylpenicillin, oxacillin, cloxacillin, nafcillin and dicloxacillin in bovine tissues by liquid chromatography-electrospray ionization tandem mass spectrometry [J].

Ito, Y ;

Ikai, Y ;

Oka, H ;

Matsumoto, H ;

Miyazaki, Y ;

Takeba, K ;

Nagase, H .

JOURNAL OF CHROMATOGRAPHY A, 2001, 911 (02) :217-223

[7]

Juhel-Gaugain M, 1999, J AOAC INT, V82, P1046

[8] Simultaneous determination of seven penicillins in muscle, liver and kidney tissues from cattle and pigs by a multiresidue high-performance liquid chromatographic method [J].

Sorensen, LK ;

Snor, LK ;

Elkær, T ;

Hansen, H .

JOURNAL OF CHROMATOGRAPHY B, 1999, 734 (02) :307-318

[9]

Verdon E, 1999, J AOAC INT, V82, P1083

← 1 →