Hydrolyzabilities of Different Corn Stover Fractions After Aqueous Ammonia Pretreatment

被引：14

作者：

Sun, Zongping ^{[1
]}

Ge, Xiaoyan ^{[1
]}

Xin, Donglin ^{[1
]}

Zhang, Junhua ^{[1
]}

机构：

[1] Northwest A&F Univ, Coll Forestry, Yangling 712100, Peoples R China

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2014年 / 172卷 / 03期

关键词：

Corn stover fractions; Aqueous ammonia pretreatment; Enzymatic hydrolysis; Cellulases; Xylanase; ENZYMATIC-HYDROLYSIS; GLUCOSE-PRODUCTION; WHEAT-STRAW; CELLULOSE; DIGESTIBILITY; TECHNOLOGIES; SOAKING; LIGNIN; XYLAN; SUGAR;

D O I：

10.1007/s12010-013-0643-y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of aqueous ammonia pretreatment on the hydrolysis of different corn stover fractions (rind, husk, leaf, and pith) by xylanase (XYL) with cellulases (CELs) was evaluated. The aqueous ammonia pretreatment had excellent delignification ability (above 66 %) for different corn stover fractions. The corn rind exhibited the lowest susceptibility to aqueous ammonia pretreatment. The pretreated rind showed the lowest hydrolyzability by CEL and XYL, which was supported by a high content of crystalline cellulose in the hydrolyzed residues of rind, as confirmed by X-ray diffraction (XRD). With the addition of 1 mg XYL/g dry matter, a high glucose yield (above 90 %) could be obtained from the pretreated rind by CEL. The results revealed that a high hydrolyzate yield of corn rind after aqueous ammonia pretreatment could be obtained with 1 mg xylanase/g dry matter, showing that aqueous ammonia pretreatment and xylanase addition to cellulases have great potential for the efficient hydrolysis of corn stover without previous fractionation.

引用

页码：1506 / 1516

页数：11

共 28 条

[1] Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: A review [J].

Alvira, P. ;

Tomas-Pejo, E. ;

Ballesteros, M. ;

Negro, M. J. .

BIORESOURCE TECHNOLOGY, 2010, 101 (13) :4851-4861

[2] Optimization of enzyme complexes for lignocellulose hydrolysis [J].

Berlin, Alex ;

Maximenko, Vera ;

Gilkes, Neil ;

Saddler, Jack .

BIOTECHNOLOGY AND BIOENGINEERING, 2007, 97 (02) :287-296

[3] Lignin in straw of herbaceous crops [J].

Buranov, Anvar U. ;

Mazza, G. .

INDUSTRIAL CROPS AND PRODUCTS, 2008, 28 (03) :237-259

[4]

Crofcheck CL, 2004, T ASAE, V47, P841, DOI 10.13031/2013.16081

[5] The maize primary cell wall microfibril: A new model derived from direct visualization [J].

Ding, SY ;

Himmel, ME .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2006, 54 (03) :597-606

[6] Effect of anatomical fractionation on the enzymatic hydrolysis of acid and alkaline pretreated corn stover [J].

Duguid, K. B. ;

Montross, M. D. ;

Radtke, C. W. ;

Crofcheck, C. L. ;

Wendt, L. M. ;

Shearer, S. A. .

BIORESOURCE TECHNOLOGY, 2009, 100 (21) :5189-5195

[7] Structural and Thermal Characterization of Wheat Straw Pretreated with Aqueous Ammonia Soaking [J].

Gao, Allan H. ;

Bule, Mahesh V. ;

Laskar, Dhrubojyoti D. ;

Chen, Shulin .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2012, 60 (35) :8632-8639

[8] Optimizing harvest of corn stover fractions based on overall sugar yields following ammonia fiber expansion pretreatment and enzymatic hydrolysis [J].

Garlock, Rebecca J. ;

Chundawat, Shishir P. S. ;

Balan, Venkatesh ;

Dale, Bruce E. .

BIOTECHNOLOGY FOR BIOFUELS, 2009, 2 :1-14

[9] THE EFFECT OF PORE-SIZE DISTRIBUTION ON THE RATE OF ENZYMATIC-HYDROLYSIS OF CELLULOSIC SUBSTRATES [J].

GRETHLEIN, HE .

BIO-TECHNOLOGY, 1985, 3 (02) :155-160

[10] Pretreatment of corn stover by soaking in aqueous ammonia at moderate temperatures [J].

Kim, Tae Hyun ;

Lee, Y. Y. .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2007, 137 (1-12) :81-92

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