Melatonin treatment further improves adipose-derived mesenchymal stem cell therapy for acute interstitial cystitis in rat

被引：68

作者：

Chen, Yen-Ta ^{[1
,2
]}

Chiang, Hsin-Ju ^{[2
,3
]}

Chen, Chih-Hung ^{[2
,4
]}

Sung, Pei-Hsun ^{[2
,5
]}

Lee, Fan-Yen ^{[2
,6
]}

Tsai, Tzu-Hsien ^{[2
,5
]}

Chang, Chia-Lo ^{[2
,7
]}

Chen, Hong-Hwa ^{[2
,7
]}

Sun, Cheuk-Kwan ^{[8
]}

Leu, Steve ^{[9
]}

Chang, Hsueh-Wen ^{[2
,10
]}

Yang, Chih-Chao ^{[2
,11
]}

Yip, Hon-Kan ^{[2
,5
,9
,12
]}

机构：

[1] Kaohsiung Chang Gung Mem Hosp, Dept Surg, Div Urol, Kaohsiung 83301, Taiwan

[2] Chang Gung Univ Coll Med, Kaohsiung, Taiwan

[3] Kaohsiung Chang Gung Mem Hosp, Dept Obstet & Gynecol, Kaohsiung 83301, Taiwan

[4] Kaohsiung Chang Gung Mem Hosp, Div Gen Med, Dept Internal Med, Kaohsiung 83301, Taiwan

[5] Kaohsiung Chang Gung Mem Hosp, Div Cardiol, Dept Internal Med, Kaohsiung 83301, Taiwan

[6] Kaohsiung Chang Gung Mem Hosp, Div Thorac & Cardiovasc Surg, Dept Surg, Kaohsiung 83301, Taiwan

[7] Kaohsiung Chang Gung Mem Hosp, Div Colorectal Surg, Dept Surg, Kaohsiung 83301, Taiwan

[8] I Shou Univ, E DA Hosp, Dept Emergency Med, Kaohsiung, Taiwan

[9] Natl Sun Yat Sen Univ, Dept Biol Sci, Kaohsiung 80424, Taiwan

[10] Kaohsiung Chang Gung Mem Hosp, Ctr Translat Res Biomed Sci, Kaohsiung 83301, Taiwan

[11] Kaohsiung Chang Gung Mem Hosp, Div Nephrol, Dept Internal Med, Kaohsiung 83301, Taiwan

[12] Kaohsiung Chang Gung Mem Hosp, Inst Shock Wave Med & Tissue Engn, Kaohsiung 83301, Taiwan

来源：

JOURNAL OF PINEAL RESEARCH | 2014年 / 57卷 / 03期

关键词：

acute interstitial cystitis; adipose-derived mesenchymal stem cells; cyclophosphamide; inflammation; oxidative stress; melatonin; ISCHEMIA-REPERFUSION INJURY; OXIDATIVE STRESS; KIDNEY INJURY; TRANSPLANTATION; EPIDEMIOLOGY;

D O I：

10.1111/jpi.12164

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

This study tests the hypothesis that combined melatonin and adipose-derived mesenchymal stem cell (ADMSC, 1.2 x 10(6) given intravenously) treatment offer superior protection against cyclophosphamide (CYP 150 mg/kg)-induced acute interstitial cystitis (AIC) in rats. Male adult Sprague-Dawley rats were treated as follows: sham controls, AIC alone, AIC + melatonin, AIC + ADMSC, and AIC + melatonin + ADMSC. When melatonin was used, it was given as follows: 20 mg/kg at 30 min after CYP and 50 mg/kg at 6 and 18 hr after CYP. Twenty-four-hour urine volume, urine albumin level, and severity of hematuria were highest in AIC rats and lowest in the controls; likewise urine volume was higher in AIC + melatonin rats than in AIC + ADMSC and AIC + melatonin + ADMSC treated rats; in all cases, P < 0.001. The numbers of CD14+, CD74+, CD68+, MIP+, Cox-2+, substance P+, cells and protein expression of IL-6, IL-12, RANTES, TNF-alpha, NF-kappa B, MMP-9, iNOS (i.e. inflammatory biomarkers), glycosaminoglycan level, expression of oxidized protein, and protein expression of reactive oxygen species (NOX-1, NOX-2, NOX-4) in the bladder tissue exhibited an identical pattern compared with that of hematuria among the five groups (all P < 0.0001). The integrity of epithelial layer and area of collagen deposition displayed an opposite pattern compared to that of hematuria among all groups (P < 0.0001). The cellular expressions of antioxidants (GR, GPx, HO-1, NQO 1) showed a significant progressive increase form controls to AIC + melatonin + ADMSC (all P < 0.0001). Combined regimen of melatonin and ADMSC was superior to either alone in protecting against CYP-induced AIC.

引用

页码：248 / 261

页数：14

共 31 条

[1] T cells from autoimmune patients display reduced sensitivity to immunoregulation by mesenchymal stem cells: Role of IL-2 [J].