Cytotoxic property of Streptococcus mitis strain producing two different types of cholesterol-dependent cytolysins

被引：6

作者：

Tabata, Atsushi ^{[1
,2
]}

Ohkuni, Hisashi ^{[3
]}

Hino, Haruka ^{[2
]}

Saigo, Takuya ^{[2
]}

Kodama, Chihiro ^{[2
]}

Tang, Qing ^{[2
]}

Tomoyasu, Toshifumi ^{[1
,2
]}

Fukunaga, Yoshitaka ^{[4
]}

Itoh, Yasuhiko ^{[4
]}

Nagamune, Hideaki ^{[1
,2
]}

机构：

[1] Univ Tokushima, Grad Sch Technol Ind & Social Sci, Dept Biosci & Bioind, 2-1 Minamijousanjima Cho, Tokushima 7708513, Japan

[2] Univ Tokushima, Inst Sci & Technol, Dept Biol Sci & Technol, Life Syst, 2-1 Minamijousanjima Cho, Tokushima 7708513, Japan

[3] Hlth Sci Res Inst East Japan, 3-673 Tenjin, Saitama 3658585, Japan

[4] Nippon Med Sch, Dept Pediat, Bunkyo Ku, 1-1-5 Sendagi, Tokyo 1138603, Japan

来源：

INFECTION GENETICS AND EVOLUTION | 2020年 / 85卷

关键词：

Streptococcus mitis; Cholesterol-dependent cytolysin; Sm-hPAF; Mitilysin; Hemolysis; Cytotoxicity; HIGH-LEVEL RESISTANCE; EXTRACELLULAR PRODUCTS; LISTERIOLYSIN-O; SP NOV; IDENTIFICATION; ENDOCARDITIS; PNEUMONIAE; PENICILLIN; TIGURINUS; MEMBER;

D O I：

10.1016/j.meegid.2020.104483

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Streptococcus mitis strain Nm-65 secretes an atypical 5-domain-type cholesterol-dependent cytolysin (CDC) called S. mitis-derived human platelet aggregation factor (Sm-hPAF) originally described as a platelet aggregation factor. Sm-hPAF belongs to Group III CDC that recognize both membrane cholesterol and human CD59 as the receptors, and shows preferential activity towards human cells. Draft genome analyses have shown that the Nm65 strain also harbors a gene encoding another CDC called mitilysin (MLY). This CDC belongs to Group I CDC that recognizes only membrane cholesterol as a receptor, and it is a homolog of the pneumococcal CDC, pneumolysin. The genes encoding each CDC are located about 20 kb apart on the Nm-65 genome. Analysis of the genomic locus of these CDC-encoding genes in silico showed that the gene encoding Sm-hPAF and the region including the gene encoding MLY were both inserted into a specific locus of the S. mitis genome. The results obtained using deletion mutants of the gene(s) encoding CDC in Nm-65 indicated that each CDC contributes to both hemolysis and cytotoxicity, and that MLY is the major hemolysin/cytolysin in Nm-65. The present study aimed to determine the potential pathogenicity of an S. mitis strain that produces two CDC with different receptor recognition properties and secretion modes.

引用

页数：8

共 46 条

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