Immobilization of Penicillium lilacinum dextranase to produce isomaltooligosaccharides from dextran

被引：31

作者：

Aslan, Yakup ^{[1
]}

Tanriseven, Aziz

机构：

[1] Harran Univ, Fac Art & Sci, Dept Biochem, TR-63300 Sanliurfa, Turkey

[2] Gebze Inst Technol, Dept Biochem, TR-41420 Kocaeli, Turkey

来源：

BIOCHEMICAL ENGINEERING JOURNAL | 2007年 / 34卷 / 01期

关键词：

immobilization; Penicillium lilacinum; dextranase; Eupergit C; isomaltooligosaccharides;

D O I：

10.1016/j.bej.2006.11.008

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Penicillium lilacinum dextranase was immobilized covalently onto Eupergit C and was used for the production of isomaltooligosaccharides. Immobilization resulted in 90% relative activity. The immobilized enzyme showed no decrease in activity for 20 batch reactions. Optimum conditions were not affected by immobilization, and optimum pH and temperature for free and immobilized enzyme were 4.5-5.5 and 30-35 degrees C, respectively. Immobilized enzyme was more stable at low and high pH and high temperatures. The kinetic parameters for the free and immobilized enzyme were also determined. The immobilized enzyme could be used for the production of isomaltooligosaccharides, since the immobilization efficiency is high (90%) and immobilized enzyme retains its activity for 20 days without any decrease. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：8 / 12

页数：5

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