Preliminary Recognition of c-Myc Gene Protein Using an Optical Biosensor with Gold Colloid Nanoparticles Based on Localized Surface Plasmon Resonance

被引：9
|
作者：
Cao, Zhong [1 ]
Gong, Fu-Chun [1 ]
Tu, Ming [1 ]
Zeng, Meng-Xue [1 ]
Huang, Xi-Xi [1 ]
Zhang, Ling [1 ]
Tan, Shu-Zhen [1 ]
Sun, Li-Xian [2 ]
Gu, Ning [3 ]
机构：
[1] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China
[2] Chinese Acad Sci, Dalian Inst Chem Phys, Mat & Thermochem Lab, Dalian, Peoples R China
[3] Southeast Univ, Sch Biol Sci & Med Engn, Natl Lab Mol & Biomol Elect, Jiangsu Lab Biomat & Devices, Nanjing, Peoples R China
来源：
ANALYTICAL LETTERS | 2009年 / 42卷 / 17期
基金：
中国国家自然科学基金; 芬兰科学院;
关键词：
Gold nanoparticles; localized surface plasmon resonance; oncogene protein; optical nanobiosensor; pCMV-Myc; ENHANCED RAMAN-SCATTERING; MICROFLUIDIC BEADS ARRAY; SILVER NANOPARTICLES; G-QUADRUPLEX; MOLECULES; ONCOGENE; SENSOR; ASSAY; METALLOPORPHYRIN; SPECTROSCOPY;
D O I：
10.1080/00032710903082820
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The gold colloidal nanoparticles with a diameter of 24 +/- 0.2nm were prepared, characterized by ultraviolet-visible (UV-vis), transmission electron microscopy (TEM), and cyclic voltammetry, and assembled on a gold plate substrate for constructing an optical nanobiosensor. The nanosensor exhibited distinct optical properties of localized surface plasmon resonance (LSPR), which could be used for recognition of oncogene biomolecules such as the c-Myc (3C7) antibody protein. The LSPR nanobiosensor has also been successfully applied to determination of a pCMV-Myc mammalian expression vector, one kind of DNA plasmids with a linear response range of 6.2-20.0ng/mu L and a detection limit of 2.4ng/mu L.
引用
收藏
页码：2820 / 2837
页数：18
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