Extracting meaningful circuit-based calcium dynamics in astrocytes and neurons from adult mouse brain slices using single-photon GCaMP imaging

Confocal, multiphoton, or other advanced microscopy techniques produce high -quality datasets of calcium activity in live tissue. However, researchers without access to such expensive equipment can still produce meaningful observations from single-photon datasets. Here, we describe a protocol to extract meaningful features of both somatic neuronal and membranous astrocytic calcium dynamics obtained from charge-coupled device (CCD)-based camera setups, typical of electrophysiology rigs and highly relevant for investigating neuronal and astro-cy tic involvement in brain circuitry. For complete details on the use and execution of this protocol, please refer to Asrican et al. (2020).