Electrochemical determination of Salmonella typhimurium by using aptamer-loaded gold nanoparticles and a composite prepared from a metal-organic framework (type UiO-67) and graphene

被引:77
作者
Dai, Ge [1 ]
Li, Zhi [1 ]
Luo, Feifei [1 ]
Ai, Shiyun [2 ]
Chen, Bo [1 ]
Wang, Qingjiang [1 ]
机构
[1] East China Normal Univ, Sch Chem & Mol Engn, 500 Dongchuan Rd, Shanghai 200241, Peoples R China
[2] Shandong Agr Univ, Coll Chem & Mat Sci, Tai An 271018, Shandong, Peoples R China
基金
中国国家自然科学基金;
关键词
Foodborne pathogens; Signal-off; Competitive; Zr-MOF; In-situ synthesis; Milk; Differential pulse voltammetry; Cyclic voltammetry; Electrochemical impedance spectroscopy; Horseradish peroxidase; GLASSY-CARBON ELECTRODE; IMPEDIMETRIC BIOSENSOR; IMMUNOSENSOR; ADSORPTION; APTASENSOR; SILICA; OXIDE;
D O I
10.1007/s00604-019-3724-y
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
An aptamer based assay is described for the determination of Salmonella typhimurium (S.typhimurium). A metal-organic framework-graphene composite of type UiO-67/GR is used as the substrate, and an aptamer-gold nanoparticles-horseradish peroxidase (Apt-AuNP-HRP) conjugate the signal amplification probe. A phosphate-terminal and partially complementary DNA (cDNA) of the aptamer is covalently bound to UiO-67/GR via the chemical complexation between phosphate and Zr-OH groups of UiO-67, and then S. typhimurium and cDNA will compete for the binding sites. The binding of Apt-AuNP-HRP to S.typhimurium leads to the formation of strong conjugates. The unbound signal probes then attach to the surface of a glassy carbon electrode via hybridization with cDNA. This generates a large current response (best measured at a potential as low as -0.02 V vs. saturated calomel electrode) under the catalytic action of HRP on the H2O2-hydroquinone system. Under the optimal conditions, the differential pulse voltammetric signal decreases linearly in the 2 x 10(1) - 2 x 10(8) cfu center dot mL(-1) S.typhimurium concentration range, with a lower detection limit of 5 cfu center dot mL(-1) (based on S/N = 3). The method was successfully applied to the detection of S. typhimurium in spiked milk samples. Graphical abstract Schematic presentation of electrochemical determination of Salmonella typhimurium(S.typhimurium). A metal-organic framework (type UiO-67) and graphene (GR) composite were used as substrate, and gold nanoparticles carrying horseradish peroxidase (HRP) for signal amplification. HQ: hydroquinone; cDNA: complementary DNA of aptamer.
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页数:9
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