Heterogeneity of proteins expressed by Brazilian Sporothrix schenckii isolates

被引：15

作者：

Fernandes, Geisa Ferreira ^{[1
]}

do Amaral, Cristiane Candida ^{[1
]}

Sasaki, Alexandre ^{[1
]}

Martinez Godoy, Patricio ^{[2
,3
]}

Pires de Camargo, Zoilo ^{[1
]}

机构：

[1] Univ Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Cellular Biol Sector, BR-04023062 Sao Paulo, Brazil

[2] Univ Fed Sao Paulo, Infect & Parasit Dis Sector, BR-04023062 Sao Paulo, Brazil

[3] Univ Austral Chile, IMC, Valdivia, Chile

来源：

MEDICAL MYCOLOGY | 2009年 / 47卷 / 08期

基金：

巴西圣保罗研究基金会;

关键词：

Sporothrix schenckii; exoantigens; proteins; SDS-PAGE; SPOROTRICHOSIS; IDENTIFICATION; PHYLOGENY; PATTERNS;

D O I：

10.3109/13693780802713216

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The profiles of proteins present in the exoantigens of Brazilian Sporothrix schenckii isolates were studied and compared by electrophoresis (SDS-PAGE). Thirteen isolates from five different regions of Brazil (1,000 to 2,000 km apart) and ten from a more limited region (200 to 400 km apart within the state of Sao Paulo) were cultured in Sabouraud, M199 and minimum (MM) media. Qualitative and quantitative differences in the expression of proteins, which varied according to the medium and the isolate, were observed. Fractions with the same MW but varying in intensity were detected, as well as fractions present in 1 isolate but absent in others. Dendrograms were constructed and isolates grouped based on the fractions obtained, irrespective of the intensity. The results showed that Brazilian S. schenckii isolates express different protein profiles, a feature also present in isolates from a more restricted region. The exoantigens were found to have a maximum of 15 protein fractions, ranging in MW from 19-220 KDaltons depending on the medium used for the cultures. These data show the great heterogeneity of Brazilian S. schenckii protein expression.

引用

页码：855 / 861

页数：7

共 31 条

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