ICAM-1 Clustering on Endothelial Cells Recruits VCAM-1

被引：43

作者：

van Buul, Jaap D. ^{[1
]}

van Rijssel, Jos ^{[1
]}

van Alphen, Floris P. J. ^{[1
]}

van Stalborch, Anna-Marieke ^{[1
]}

Mul, Erik P. J. ^{[1
]}

Hordijk, Peter L. ^{[1
]}

机构：

[1] Univ Amsterdam, Dept Mol Cell Biol, Sanquin Res & Landsteiner Lab, Acad Med Ctr, NL-1066 CX Amsterdam, Netherlands

来源：

JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY | 2010年

关键词：

E-SELECTIN; ADHESION; TETRASPANINS; ACTIVATION; CONTACTS;

D O I：

10.1155/2010/120328

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In the initial stages of transendothelial migration, leukocytes use the endothelial integrin ligands ICAM-1 and VCAM-1 for strong adhesion. Upon adhesion of the leukocyte to endothelial ICAM-1, ICAM-1 is clustered and recruited to the adhered leukocyte, promoting strong adhesion. In this study, we provide evidence for the colocalization of VCAM-1 at sites of ICAM-1 clustering. Anti-ICAM-1 antibody-coated beads were used to selectively cluster and recruit ICAM-1 on primary human endothelial cells. In time, co-localization of ICAM-1 and VCAM-1 around the adherent beads was observed. Biochemical pull-down assays showed that ICAM-1 clustering induced its association to VCAM-1, suggesting a physical link between these two adhesion molecules. The association was partly dependent on lipid rafts as well as on F-actin and promoted adhesion. These data show that VCAM-1 can be recruited, in an integrin-independent fashion, to clustered ICAM-1 which may serve to promote ICAM-1-mediated leukocyte adhesion.

引用

页数：9

共 23 条

[1] ICAM-1-mediated, Src- and Pyk2-dependent vascular endothelial cadherin tyrosine phosphorylation is required for leukocyte transendothelial migration [J].