Human type I cytokeratin genes are a compact cluster

被引：15
作者：
Ceratto, N
Dobkin, C
Carter, M
Jenkins, E
Yao, XL
Cassiman, JJ
Aly, MS
Bosco, P
Leube, R
Langbein, L
Feo, S
Romano, V
机构：
[1] IRCCS,IST RIC RITARDO MENTALE & INVOLUZ CEREBRALE,OASI,MOL GENET LAB,I-94018 TROINA,EN,ITALY
[2] NEW YORK STATE INST BASIC RES DEV DISABIL,DEPT HUMAN GENET,STATEN ISL,NY 10314
[3] CATHOLIC UNIV LEUVEN,CTR HUMAN GENET,B-3000 LOUVAIN,BELGIUM
[4] UNIV MAINZ,FAC MED,INST ANAT,D-6500 MAINZ,GERMANY
[5] GERMAN CANC RES CTR,INST CELL & TUMOR BIOL,D-6900 HEIDELBERG,GERMANY
[6] UNIV PALERMO,DIPARTIMENTO BIOL CELLULARE & SVILUPPO,PALERMO,ITALY
来源：
CYTOGENETICS AND CELL GENETICS | 1997年 / 77卷 / 3-4期
关键词：
D O I：
10.1159/000134566
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
A YAC clone (211F11) containing approximately 0.5 Mb of human DNA was isolated from a human genomic library by PCR-based screening with cytokeratin (KRT) 13-specific primers. The YAC clone was mapped by FISH to the long arm of chromosome 17 (17q12-->q21), a region to which several other type I KRT genes had been mapped previously. We now show by Southern blot hybridization and PFGE analyses that KRT13, 14, 15, and 16 are all contained within YAC clone 211F11. Long-range restriction mapping analysis of clone 211F11 and of two smaller YAC clones that were also isolated with KRT13-specific primers, suggests that KRT13, 14, 15, 16 and their linked type I genes KRT17 and 19, are contained in less than 150 kb of genomic DNA. According to our reconstruction it then appears that at least six type I KRT genes are arranged in a highly compact cluster. The three YACs reported in this study represent a new tool to dissect the molecular structure of the locus of the human type I KRT genes.
引用
收藏
页码：169 / 174
页数：6
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