Microorganisms linked to inflammatory bowel disease-associated dysbiosis differentially impact host physiology in gnotobiotic mice

被引:112
作者
Hoffmann, Thomas W. [1 ,2 ]
Hang-Phuong Pham [3 ]
Bridonneau, Chantal [1 ,2 ]
Aubry, Camille [1 ,2 ]
Lamas, Bruno [4 ]
Martin-Gallausiaux, Camille [1 ,2 ]
Moroldo, Marco [5 ,6 ]
Rainteau, Dominique [4 ]
Lapaque, Nicolas [1 ,2 ]
Six, Adrien [7 ]
Richard, Mathias L. [1 ,2 ]
Fargier, Emilie [8 ]
Le Guern, Marie-Emmanuelle [8 ]
Langella, Philippe [1 ,2 ]
Sokol, Harry [1 ,2 ,4 ,9 ,10 ]
机构
[1] INRA, MICALIS UMR1319, Jouy En Josas, France
[2] AgroParisTech, MICALIS UMR1319, Jouy En Josas, France
[3] Hop La Pitie Salpetriere, ILTOO Pharma, Inst Cerveau & Moelle Epiniere, Paris, France
[4] Univ Paris 06, ERL, INSERM, Fac Med St Antoine,UMR7203,U1157, Paris, France
[5] INRA, GABI CRB GADIE UMR1313, Jouy En Josas, France
[6] AgroParisTech, GABI UMR1313, Jouy En Josas, France
[7] Univ Paris 06, Sorbonne Univ, INSERM, I3,CNRS FRE 3632,UMR S 959, Paris, France
[8] Ctr Rech Dev, Biocodex, Compiegne, France
[9] Hop St Antoine, AP HP, Serv Gastroenterol & Nutr, 184 Rue Faubourg St Antoine, F-75571 Paris 12, France
[10] Univ Paris 06, 184 Rue Faubourg St Antoine, F-75571 Paris 12, France
关键词
ESCHERICHIA-COLI STRAINS; SACCHAROMYCES-BOULARDII; INDOLEAMINE 2,3-DIOXYGENASE; CANDIDA-ALBICANS; INTERFERON-GAMMA; ILEAL MUCOSA; MOUSE MODEL; TRYPTOPHAN CATABOLISM; HUMAN-FIBROBLASTS; DENDRITIC CELLS;
D O I
10.1038/ismej.2015.127
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
Studying host-microbiota interactions are fundamental to understanding the mechanisms involved in intestinal homeostasis and inflammation. In this work, we analyzed these interactions in mice that were mono-associated with six microorganisms that are representative of inflammatory bowel disease (IBD)-associated dysbiosis: the bacteria Bacteroides thetaiotaomicron, adhesive-invasive Escherichia coli (AIEC), Ruminococcus gnavus and Roseburia intestinalis; a yeast used as a probiotic drug, Saccharomyces boulardii CNCM I-745; and another yeast, Candida albicans. Extensive ex vivo analyses including colon transcriptomics, histology, immune response, bile acid metabolism and short-chain fatty acid production were studied. We showed that B. thetaiotaomicron had the highest impact on the immune system because it was almost able to recapitulate the effects of the entire conventional microbiota and notably induced Treg pathways. Furthermore, these analyses uncovered the effects of E. coli AIEC LF82 on indoleamine 2,3-dioxygenase expression and of S. boulardii CNCM I-745 on angiogenesis. These results were confirmed in vitro in human cell lines. Finally, our results suggested that R. gnavus has major effects on metabolism, and notably on tryptophan metabolism. This work therefore reveals that microorganisms with a potential role in intestinal homeostasis and inflammation have specific impacts on the host, and it suggests several tracks to follow to understand intestinal homeostasis and IBD pathogenesis better, providing new insights to identify novel therapeutic targets.
引用
收藏
页码:460 / 477
页数:18
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