Biological Effect of Different Spinach Extracts in Comparison with the Individual Components of the Phytocomplex

被引:12
作者
Arru, Laura [1 ,2 ]
Mussi, Francesca [1 ,3 ]
Forti, Luca [1 ]
Buschini, Annamaria [3 ,4 ]
机构
[1] Univ Modena & Reggio Emilia, Dept Life Sci, I-41125 Modena, Italy
[2] Univ Modena & Reggio Emilia, Int Ctr BIOGEST SITEIA, I-42122 Reggio Emilia, Italy
[3] Univ Parma, Dept Chem Life Sci & Environm Sustainabil, I-43124 Parma, Italy
[4] Univ Parma, Int Ctr COMT, I-43124 Parma, Italy
关键词
phytocomplex; spinach extracts; colon cancer cell line; phytochemicals; antioxidants;
D O I
10.3390/foods10020382
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The Mediterranean-style diet is rich in fruit and vegetables and has a great impact on the prevention of major chronic diseases, such as cardiovascular diseases and cancer. In this work we investigated the ability of spinach extracts obtained by different extraction methods and of the single main components of the phytocomplex, alone or mixed, to modulate proliferation, antioxidant defense, and genotoxicity of HT29 human colorectal cells. Spinach extracts show dose-dependent activity, increasing the level of intracellular endogenous reactive oxygen species (ROS) when tested at higher doses. In the presence of oxidative stress, the activity is related to the oxidizing agent involved (H2O2 or menadione) and by the extraction method. The single components of the phytocomplex, alone or mixed, do not alter the intracellular endogenous level of ROS but again, in the presence of an oxidative insult, the modulation of antioxidant defense depends on the oxidizing agent used. The application of the phytocomplex extracts seem to be more effective than the application of the single phytocomplex components.
引用
收藏
页码:1 / 15
页数:15
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