Inhibitory Effects of Gold and Silver Nanoparticles on the Differentiation into Osteoclasts In Vitro

被引：18

作者：

Lee, Daye ^{[1
,2
]}

Ko, Wan-Kyu ^{[1
,2
]}

Kim, Seong Jun ^{[1
,2
]}

Han, In-Bo ^{[1
]}

Hong, Je Beom ^{[3
]}

Sheen, Seung Hun ^{[1
]}

Sohn, Seil ^{[1
]}

机构：

[1] CHA Univ, Dept Neurosurg, CHA Bundang Med Ctr, 59 Yatap Ro, Seongnam Si 13496, Gyeonggi Do, South Korea

[2] CHA Univ, Dept Biomed Sci, Seongnam Si 13496, Gyeonggi Do, South Korea

[3] Sungkyunkwan Univ, Sch Med, Kangbuk Samsung Hosp, Dept Neurosurg, Seoul 03181, South Korea

来源：

PHARMACEUTICS | 2021年 / 13卷 / 04期

基金：

新加坡国家研究基金会;

关键词：

gold nanoparticles; silver nanoparticles; osteoclast; bone marrow-derived macrophages; RANKL;

D O I：

10.3390/pharmaceutics13040462

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Gold nanoparticles (GNPs) have been widely studied to inhibit differentiation into osteoclasts. However, reports of the inhibitory effects of silver nanoparticles (SNPs) during the process of differentiation into osteoclasts are rare. We compared the inhibitory effect of GNPs and SNPs during the process of differentiation into osteoclasts. Bone marrow-derived cells were differentiated into osteoclasts by the receptor activator of the nuclear factor-kappa-Beta ligand (RANKL). The inhibitory effect of GNPs or SNPs during the process of differentiation into osteoclasts was investigated using tartrate-resistant acid phosphatase (TRAP) and actin ring staining. The formation of TRAP positive ((+)) multinuclear cells (MNCs) with the actin ring structure was most inhibited in the SNP group. In addition, the expression of specific genes related to the differentiation into osteoclasts, such as c-Fos, the nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1), TRAP, and Cathepsin K (CTSK) were also inhibited in the SNP groups. As a result, the levels related to differentiation into osteoclasts were consistently lower in the SNP groups than in the GNP groups. Our study suggests that SNPs can be a useful material for inhibiting differentiation into osteoclasts and they can be applied to treatments for osteoporosis patients.

引用

页数：13

共 49 条

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