Combined photoacoustic and ultrasound biomicroscopy

被引：77

作者：

Harrison, Tyler ^{[1
]}

Ranasinghesagara, Janaka C. ^{[1
]}

Lu, Huihong ^{[1
]}

Mathewson, Kory ^{[1
]}

Walsh, Andrew ^{[1
]}

Zemp, Roger J. ^{[1
]}

机构：

[1] Univ Alberta, Dept Elect & Comp Engn, Edmonton, AB T6G 2V4, Canada

来源：

OPTICS EXPRESS | 2009年 / 17卷 / 24期

基金：

加拿大创新基金会; 加拿大自然科学与工程研究理事会;

关键词：

IN-VIVO; HIGH-RESOLUTION; MICROSCOPY;

D O I：

10.1364/OE.17.022041

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We report on the development of an imaging system capable of combined ultrasound and photoacoustic imaging based on a fast-scanning single-element 25-MHz ultrasound transducer and a unique light-delivery system. The system is capable of 20 ultrasound frames per second and slower photoacoustic frame rates limited by laser pulse-repetition rates. Laser and ultrasound pulses are interlaced for co-registration of photoacoustic and ultrasound images. In vivo imaging of a human finger permits ultrasonic visualization of vessel structures and speckle changes indicative of blood flow, while overlaid photoacoustic images highlight some small vessels that are not clear from the ultrasound scan. Photoacoustic images provide optical absorption contrast co-registered in the structural and blood-flow context of ultrasound with high-spatial resolution and may prove important for clinical diagnostics and basic science of the microvasculature. (C) 2009 Optical Society of America

引用

页码：22041 / 22046

页数：6

共 10 条

[1] A new ultrasound instrument for in vivo microimaging of mice [J].

Foster, FS ;

Zhang, MY ;

Zhou, YQ ;

Liu, G ;

Mehi, J ;

Cherin, E ;

Harasiewicz, KA ;

Starkoski, BG ;

Zan, L ;

Knapik, DA ;

Adamson, SL .

ULTRASOUND IN MEDICINE AND BIOLOGY, 2002, 28 (09) :1165-1172

[2] Functional transcranial brain imaging by optical-resolution photoacoustic microscopy [J].

Hu, Song ;

Maslov, Konstantin ;

Tsytsarev, Vassiliy ;

Wang, Lihong V. .

JOURNAL OF BIOMEDICAL OPTICS, 2009, 14 (04)

[3] SIMULTANEOUS IMAGING OF A lacZ-MARKED TUMOR AND MICROVASCULATURE MORPHOLOGY IN VIVO BY DUAL-WAVELENGTH PHOTOACOUSTIC MICROSCOPY [J].

Li, Li ;

Zhang, Hao F. ;

Zemp, Roger J. ;

Maslov, Konstantin ;

Wang, Lihong V. .

JOURNAL OF INNOVATIVE OPTICAL HEALTH SCIENCES, 2008, 1 (02) :207-215

[4] Effects of wavelength-dependent fluence attenuation on the noninvasive photoacoustic imaging of hemoglobin oxygen saturation in subcutaneous vasculature in vivo [J].

Maslov, Konstantin ;

Zhang, Hao F. ;

Wang, Lihong V. .

INVERSE PROBLEMS, 2007, 23 (06) :S113-S122

[5] Combined ultrasound and optoacoustic system for real-time high-contrast vascular imaging in vivo [J].

Niederhauser, JJ ;

Jaeger, M ;

Lemor, R ;

Weber, P ;

Frenz, M .

IEEE TRANSACTIONS ON MEDICAL IMAGING, 2005, 24 (04) :436-440

[6] Prospects of photoacoustic tomography [J].

Wang, Lihong V. .

MEDICAL PHYSICS, 2008, 35 (12) :5758-5767

[7] Photoacoustic imaging in biomedicine [J].

Xu, MH ;

Wang, LHV .

REVIEW OF SCIENTIFIC INSTRUMENTS, 2006, 77 (04)

[8] Realtime photoacoustic microscopy in vivo with a 30-MHz ultrasound array transducer [J].

Zemp, Roger J. ;

Song, Liang ;

Bitton, Rachel ;

Shung, K. Kirk ;

Wang, Lihong V. .

OPTICS EXPRESS, 2008, 16 (11) :7915-7928

[9] Backward-mode multiwavelength photoacoustic scanner using a planar Fabry-Perot polymer film ultrasound sensor for high-resolution three-dimensional imaging of biological tissues [J].

Zhang, Edward ;

Laufer, Jan ;

Beard, Paul .

APPLIED OPTICS, 2008, 47 (04) :561-577

[10] Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging [J].

Zhang, Hao F. ;

Maslov, Konstantin ;

Stoica, George ;

Wang, Lihong V. .

NATURE BIOTECHNOLOGY, 2006, 24 (07) :848-851

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