A new chemical approach for proximity labelling of chromatin-associated RNAs and proteins with visible light irradiation

被引：16
作者：
Li, Lan [1 ]
Liang, Jiying [1 ]
Luo, Hao [1 ]
Tam, K. Ming [1 ]
Tse, Edmund C. M. [1 ]
Li, Ying [1 ]
机构：
[1] Univ Hong Kong, Dept Chem, Pokfulam Rd, Hong Kong, Peoples R China
来源：
CHEMICAL COMMUNICATIONS | 2019年 / 55卷 / 82期
关键词：
MESSENGER-RNA; LIVE-CELL; LOCALIZATION; TRANSLATION; GENERATION; DESIGN; LINE;
D O I：
10.1039/c9cc06251c
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A new nucleus-localized singlet oxygen generator was designed and synthesized. Its capability to label nucleus-localized biomolecules through spatially-restricted oxidation under visible light was validated using fluorescence confocal microscopy. Western blot and RT-qPCR were performed to verify the desirable spatial resolution through enriching chromatin-associated RNAs and proteins.
引用
收藏
页码：12340 / 12343
页数：4
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