Glassy carbon electrodes modified with CNT dispersed in chitosan: Analytical applications for sensing DNA-methylene blue interaction

被引：48

作者：

Arias, Paulina ^{[1
]}

Ferreyra, Nancy F. ^{[2
]}

Rivas, Gustavo A. ^{[2
]}

Bollo, Soledad ^{[1
]}

机构：

[1] Univ Chile, Fac Ciencias Quim & Farmaceut, Santiago, Chile

[2] Univ Nacl Cordoba, Fac Ciencias Quim, Dept Quim Fis, INFIQC, RA-5000 Cordoba, Argentina

来源：

JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 2009年 / 634卷 / 02期

关键词：

Carbon nanotubes; Chitosan; Glassy carbon electrode; DNA damage; Methylene blue sensor; Methylene blue electrochemistry; ELECTROCHEMICAL SENSORS; BIOSENSORS; NANOTUBES; BINDING; DRUGS;

D O I：

10.1016/j.jelechem.2009.07.022

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this work we report the optimization of a chitosan (CHI) and carbon nanotubes (CNT) modified electrode, CNT-CHI, to study the dsDNA interaction with methylene blue (MB), a well-known DNA intercalator. The analytical signal was the reduction current of the MB associated with the dsDNA confined at CNT-CHI modified glassy carbon electrode. The selected CNT-CHI film stabilizing treatment was immersion for 2 s in a 0.20% GTA solution. The effect of ionic strength was also analyzed, concluding that high concentration of NaCl (90 mM) benefits the intercalative process. Below these conditions, a linear response between intensity current and MB concentration was obtained. In conclusion. modifying the initial conditions of the assembling GCE/CHI-CNT/dsDNA electrode the intercalative process was favored opening a fast and cheap possibility to evaluate the toxicity of new synthesized molecules. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：123 / 126

页数：4

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