Direct evidence for membrane pore formation by the apoptotic protein Bax

被引：90

作者：

Epand, RF

Martinou, JC

Montessuit, S

Epand, RM ^{[1
]}

Yip, CM

机构：

[1] McMaster Univ, Hlth Sci Ctr, Dept Biochem, Hamilton, ON L8N 3Z5, Canada

[2] Univ Geneva, Dept Biol Cellulaire, CH-1211 Geneva 4, Switzerland

[3] Univ Toronto, Dept Chem Engn & Appl Chem, Inst Biomat & Biomed Engn, Toronto, ON M5S 3G9, Canada

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 298卷 / 05期

基金：

加拿大健康研究院; 加拿大自然科学与工程研究理事会;

关键词：

Bax; atomic force microscopy; membrane pore; apoptosis; calcium;

D O I：

10.1016/S0006-291X(02)02544-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Direct imaging of the interaction of the apoptotic protein, Bax, with membrane bilayers shows the presence of toroidal-shaped pores using atomic force microscopy. These pores are sufficiently large to allow passage of proteins from the intermitochondrial space. Both the perturbation of the membrane and the amount of protein bound to the bilayer are increased in the presence of calcium. The results from the imaging are consistent with leakage studies from liposomes of the same composition. The work shows that Bax by itself can form pores in membrane bilayers. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

收藏

页码：744 / 749

页数：6

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