Identification of rat serum alkaline phosphatase isoenzyme by means of wheat germ agglutinin

被引：3
|
作者：
Wada, H
Niwa, N
Hayakawa, T
Tsuge, H
机构：
[1] GIFU UNIV,FAC AGR,DEPT FOOD SCI,GIFU 50111,JAPAN
[2] GIFU UNIV,FAC AGR,UNITED GRAD SCH AGR SCI,GIFU 50111,JAPAN
来源：
EXPERIMENTAL ANIMALS | 1997年 / 46卷 / 01期
关键词：
rat; serum alkaline phosphatase (sALP); wheat germ agglutinin (WGA);
D O I：
10.1538/expanim.46.89
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Wheat germ agglutinin (WGA) precipitates bone type serum alkaline phosphatase (sALP) isoenzyme specifically. The precipitates are composed of the macromolecules of WGA and ''bone type sALP'' (WGA-ALP complex). In order to use bone type sALP as a marker in polyacrylamide gel electrophoresis (PAGE), a method to separate ''bone type sALP'' from the ''WGA-ALP complex'' was established by using N-acetyl-D-glucosamine (GlcNAc)-Sepharose 6B column chromatography. It was concluded that this method is useful for clinical examination in the rat.
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页码：89 / 92
页数：4
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