Induction of Pluripotency in Adult Unipotent Germline Stem Cells

被引：195

作者：

Ko, Kinarm ^{[1
]}

Tapia, Natalia ^{[1
]}

Wu, Guangming ^{[1
]}

Kim, Jeong Beom ^{[1
]}

Arauzo-Bravo, Marcos J. ^{[1
]}

Sasse, Philipp ^{[2
]}

Glaser, Tamara ^{[3
,4
]}

Ruau, David ^{[5
,6
]}

Han, Dong Wook ^{[1
]}

Greber, Boris ^{[1
]}

Hausdoerfer, Kirsten ^{[3
,4
]}

Sebastiano, Vittorio ^{[1
]}

Stehling, Martin ^{[1
]}

Fleischmann, Bernd K. ^{[2
]}

Bruestle, Oliver ^{[3
,4
]}

Zenke, Martin ^{[5
,6
]}

Schoeler, Hans R. ^{[1
]}

机构：

[1] Max Planck Inst Mol Biomed, Dept Cell & Dev Biol, D-48149 Munster, Germany

[2] Univ Bonn, Life & Brain Ctr, Inst Physiol 1, D-53105 Bonn, Germany

[3] Univ Bonn, Life & Brain Ctr, Inst Reconstruct Neurobiol, D-53105 Bonn, Germany

[4] Hertie Fdn, D-53105 Bonn, Germany

[5] Rhein Westfal TH Aachen, Sch Med, Dept Cell Biol, Inst Biomed Engn, D-52074 Aachen, Germany

[6] Rhein Westfal TH Aachen, Helmholtz Inst Biomed Engn, D-52074 Aachen, Germany

来源：

CELL STEM CELL | 2009年 / 5卷 / 01期

关键词：

LONG-TERM PROLIFERATION; MYELIN-DEFICIENT RAT; MOUSE TESTIS; RETINOIC ACID; SELF-RENEWAL; GENERATION; CULTURE; SPERMATOGENESIS; ESTABLISHMENT; METHYLATION;

D O I：

10.1016/j.stem.2009.05.025

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Mouse and human stem cells with features similar to those of embryonic stem cells have been derived from testicular cells. Although pluripotent stem cells have been obtained from defined germline stem cells (GSCs) of mouse neonatal testis, only multipotent stem cells have been obtained so far from defined cells of mouse adult testis. In this study we describe a robust and reproducible protocol for obtaining germ line-derived pluripotent stem (gPS) cells from adult unipotent GSCs. Pluripotency of gPS cells was confirmed by in vitro and in vivo differentiation, including germ cell contribution and transmission. As determined by clonal analyses, gPS cells indeed originate from unipotent GSCs. We propose that the conversion process requires a GSC culture microenvironment that depends on the initial number of plated GSCs and the length of culture time.

引用

页码：87 / 96

页数：10

共 50 条

[41] Patterning Pluripotency in Embryonic Stem Cells [J].

Zhang, Yue Shelby ;

Sevilla, Ana ;

Wan, Leo Q. ;

Lemischka, Ihor R. ;

Vunjak-Novakovic, Gordana .

STEM CELLS, 2013, 31 (09) :1806-1815

[42] Pluripotency and differentiation of embryonic stem cells [J].

Liu, Yinyin ;

Zhao, Haibo ;

Liang, Liang ;

Fan, Peilei ;

Zhao, Yujia ;

Feng, Jinling ;

Zhang, Ying ;

Gao, Yang ;

Shen, Zhengsheng .

2020 INTERNATIONAL CONFERENCE ON ENERGY, ENVIRONMENT AND BIOENGINEERING (ICEEB 2020), 2020, 185

[43] Prediction of novel pluripotent proteins involved in reprogramming of male Germline stem cells (GSCs) into multipotent adult Germline stem cells (maGSCs) by network analysis [J].

Guttula, Praveen Kumar ;

Agarwal, Anushka ;

Maharana, Usharani ;

Gupta, Mukesh Kumar .

COMPUTATIONAL BIOLOGY AND CHEMISTRY, 2018, 76 :302-309

[44] Homologous Recombination in Rat Germline Stem Cells [J].

Kanatsu-Shinohara, Mito ;

Kato-Itoh, Megumi ;

Ikawa, Masahito ;

Takehashi, Masanori ;

Sanbo, Makoto ;

Morioka, Yuka ;

Tanaka, Takashi ;

Morimoto, Hiroko ;

Hirabayashi, Masumi ;

Shinohara, Takashi .

BIOLOGY OF REPRODUCTION, 2011, 85 (01) :208-217

[45] Characterization of Immortalized Dairy Goat male Germline Stem Cells (mGSCs) [J].

Zhu, Haijing ;

Ma, Jing ;

Du, Rui ;

Zheng, Liming ;

Wu, Jiang ;

Song, Wencong ;

Niu, Zhiwei ;

He, Xin ;

Du, Enqi ;

Zhao, Shanting ;

Hua, Jinlian .

JOURNAL OF CELLULAR BIOCHEMISTRY, 2014, 115 (09) :1549-1560

[46] Histone methyltransferase DOT1L is essential for self-renewal of germline stem cells [J].

Lin, Huijuan ;

Cheng, Keren ;

Kubota, Hiroshi ;

Lan, Yemin ;

Riedel, Simone S. ;

Kakiuchi, Kazue ;

Sasaki, Kotaro ;

Bernt, Kathrin M. ;

Bartolomei, Marisa S. ;

Luo, Mengcheng ;

Wang, P. Jeremy .

GENES & DEVELOPMENT, 2022, 36 (11-12) :752-763

[47] Induction of Pluripotency in Astrocytes through a Neural Stem Cell-like State [J].

Nakajima-Koyama, May ;

Lee, Joonseong ;

Ohta, Sho ;

Yamamoto, Takuya ;

Nishida, Eisuke .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2015, 290 (52) :31173-31188

[48] Epiblast-like stem cells established by Wnt/(3-catenin signaling manifest distinct features of formative pluripotency and germline competence [J].

Luo, Qing ;

Pui, Han-pin ;

Chen, Jiayu ;

Yu, Leqian ;

Jannig, Paulo R. ;

Pei, Yu ;

Zhao, Linxuan ;

Chen, Xingqi ;

Petropoulos, Sophie ;

Ruas, Jorge L. ;

Wu, Jun ;

Deng, Qiaolin .

CELL REPORTS, 2023, 42 (01)

[49] Two Factor Reprogramming of Human Neural Stem Cells into Pluripotency [J].

Hester, Mark E. ;

Song, SungWon ;

Miranda, Carlos J. ;

Eagle, Amy ;

Schwartz, Phillip H. ;

Kaspar, Brian K. .

PLOS ONE, 2009, 4 (09)

[50] A Real-Time Pluripotency Reporter for Human Stem Cells [J].

Zhong, Jiang F. ;

Weiner, Leslie ;

Jin, Yangsun ;

Lu, Wange ;

Taylor, Clive R. .

STEM CELLS AND DEVELOPMENT, 2010, 19 (01) :47-52

← 1 2 3 4 5 →