Induction of Pluripotency in Adult Unipotent Germline Stem Cells

被引：195

作者：

Ko, Kinarm ^{[1
]}

Tapia, Natalia ^{[1
]}

Wu, Guangming ^{[1
]}

Kim, Jeong Beom ^{[1
]}

Arauzo-Bravo, Marcos J. ^{[1
]}

Sasse, Philipp ^{[2
]}

Glaser, Tamara ^{[3
,4
]}

Ruau, David ^{[5
,6
]}

Han, Dong Wook ^{[1
]}

Greber, Boris ^{[1
]}

Hausdoerfer, Kirsten ^{[3
,4
]}

Sebastiano, Vittorio ^{[1
]}

Stehling, Martin ^{[1
]}

Fleischmann, Bernd K. ^{[2
]}

Bruestle, Oliver ^{[3
,4
]}

Zenke, Martin ^{[5
,6
]}

Schoeler, Hans R. ^{[1
]}

机构：

[1] Max Planck Inst Mol Biomed, Dept Cell & Dev Biol, D-48149 Munster, Germany

[2] Univ Bonn, Life & Brain Ctr, Inst Physiol 1, D-53105 Bonn, Germany

[3] Univ Bonn, Life & Brain Ctr, Inst Reconstruct Neurobiol, D-53105 Bonn, Germany

[4] Hertie Fdn, D-53105 Bonn, Germany

[5] Rhein Westfal TH Aachen, Sch Med, Dept Cell Biol, Inst Biomed Engn, D-52074 Aachen, Germany

[6] Rhein Westfal TH Aachen, Helmholtz Inst Biomed Engn, D-52074 Aachen, Germany

来源：

CELL STEM CELL | 2009年 / 5卷 / 01期

关键词：

LONG-TERM PROLIFERATION; MYELIN-DEFICIENT RAT; MOUSE TESTIS; RETINOIC ACID; SELF-RENEWAL; GENERATION; CULTURE; SPERMATOGENESIS; ESTABLISHMENT; METHYLATION;

D O I：

10.1016/j.stem.2009.05.025

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Mouse and human stem cells with features similar to those of embryonic stem cells have been derived from testicular cells. Although pluripotent stem cells have been obtained from defined germline stem cells (GSCs) of mouse neonatal testis, only multipotent stem cells have been obtained so far from defined cells of mouse adult testis. In this study we describe a robust and reproducible protocol for obtaining germ line-derived pluripotent stem (gPS) cells from adult unipotent GSCs. Pluripotency of gPS cells was confirmed by in vitro and in vivo differentiation, including germ cell contribution and transmission. As determined by clonal analyses, gPS cells indeed originate from unipotent GSCs. We propose that the conversion process requires a GSC culture microenvironment that depends on the initial number of plated GSCs and the length of culture time.

引用

页码：87 / 96

页数：10

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