Induction of Pluripotency in Adult Unipotent Germline Stem Cells

被引：195

作者：

Ko, Kinarm ^{[1
]}

Tapia, Natalia ^{[1
]}

Wu, Guangming ^{[1
]}

Kim, Jeong Beom ^{[1
]}

Arauzo-Bravo, Marcos J. ^{[1
]}

Sasse, Philipp ^{[2
]}

Glaser, Tamara ^{[3
,4
]}

Ruau, David ^{[5
,6
]}

Han, Dong Wook ^{[1
]}

Greber, Boris ^{[1
]}

Hausdoerfer, Kirsten ^{[3
,4
]}

Sebastiano, Vittorio ^{[1
]}

Stehling, Martin ^{[1
]}

Fleischmann, Bernd K. ^{[2
]}

Bruestle, Oliver ^{[3
,4
]}

Zenke, Martin ^{[5
,6
]}

Schoeler, Hans R. ^{[1
]}

机构：

[1] Max Planck Inst Mol Biomed, Dept Cell & Dev Biol, D-48149 Munster, Germany

[2] Univ Bonn, Life & Brain Ctr, Inst Physiol 1, D-53105 Bonn, Germany

[3] Univ Bonn, Life & Brain Ctr, Inst Reconstruct Neurobiol, D-53105 Bonn, Germany

[4] Hertie Fdn, D-53105 Bonn, Germany

[5] Rhein Westfal TH Aachen, Sch Med, Dept Cell Biol, Inst Biomed Engn, D-52074 Aachen, Germany

[6] Rhein Westfal TH Aachen, Helmholtz Inst Biomed Engn, D-52074 Aachen, Germany

来源：

CELL STEM CELL | 2009年 / 5卷 / 01期

关键词：

LONG-TERM PROLIFERATION; MYELIN-DEFICIENT RAT; MOUSE TESTIS; RETINOIC ACID; SELF-RENEWAL; GENERATION; CULTURE; SPERMATOGENESIS; ESTABLISHMENT; METHYLATION;

D O I：

10.1016/j.stem.2009.05.025

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Mouse and human stem cells with features similar to those of embryonic stem cells have been derived from testicular cells. Although pluripotent stem cells have been obtained from defined germline stem cells (GSCs) of mouse neonatal testis, only multipotent stem cells have been obtained so far from defined cells of mouse adult testis. In this study we describe a robust and reproducible protocol for obtaining germ line-derived pluripotent stem (gPS) cells from adult unipotent GSCs. Pluripotency of gPS cells was confirmed by in vitro and in vivo differentiation, including germ cell contribution and transmission. As determined by clonal analyses, gPS cells indeed originate from unipotent GSCs. We propose that the conversion process requires a GSC culture microenvironment that depends on the initial number of plated GSCs and the length of culture time.

引用

页码：87 / 96

页数：10

共 34 条

[1]

Anderson EL, 2008, P NATL ACAD SCI USA, V105, P14976, DOI [10.1073/pnas.0807297105, 10.1038/s41598-019-45839-z]

[2] MYELIN-DEFICIENT RAT - A POINT MUTATION IN EXON-III (A-]C, THR75-]PRO) OF THE MYELIN PROTEOLIPID PROTEIN CAUSES DYSMYELINATION AND OLIGODENDROCYTE DEATH [J].

BOISON, D ;

STOFFEL, W .

EMBO JOURNAL, 1989, 8 (11) :3295-3302

[3] Embryonic stem cell-derived glial precursors:: A source of myelinating transplants [J].

Brüstle, O ;

Jones, KN ;

Learish, RD ;

Karram, K ;

Choudhary, K ;

Wiestler, OD ;

Duncan, ID ;

McKay, RDG .

SCIENCE, 1999, 285 (5428) :754-756

[4] RETRACTED: Generation of pluripotent stem cells from adult human testis (Retracted article. See vol. 512, pg. 338, 2014) [J].

Conrad, Sabine ;

Renninger, Markus ;

Hennenlotter, Joerg ;

Wiesner, Tina ;

Just, Lothar ;

Bonin, Michael ;

Aicher, Wilhelm ;

Buehring, Hans-Joerg ;

Mattheus, Ulrich ;

Mack, Andreas ;

Wagner, Hans-Joachim ;

Minger, Stephen ;

Matzkies, Matthias ;

Reppel, Michael ;

Hescheler, Juergen ;

Sievert, Karl-Dietrich ;

Stenzl, Arnulf ;

Skutella, Thomas .

NATURE, 2008, 456 (7220) :344-U25

[5] Spermatogonial Stem Cell Self-Renewal Requires OCT4, a Factor Downregulated During Retinoic Acid-Induced Differentiation [J].

Dann, Christina Tenenhaus ;

Alvarado, Alma L. ;

Molyneux, Laura A. ;

Denard, Bray S. ;

Garbers, David L. ;

Porteus, Matthew H. .

STEM CELLS, 2008, 26 (11) :2928-2937

[6] Repair of myelin disease: strategies and progress in animal models [J].

Duncan, ID ;

Grever, WE ;

Zhang, SC .

MOLECULAR MEDICINE TODAY, 1997, 3 (12) :554-561

[7]

FEIL R, 1994, DEVELOPMENT, V120, P2933

[8] Pluripotency of spermatogonial stem cells from adult mouse testis [J].

Guan, K ;

Nayernia, K ;

Maier, LS ;

Wagner, S ;

Dressel, R ;

Lee, JH ;

Nolte, J ;

Wolf, F ;

Li, MY ;

Engel, W ;

Hasenfuss, G .

NATURE, 2006, 440 (7088) :1199-1203

[9] Action potential propagation failures in long-term recordings from embryonic stem cell-derived cardiomyocytes in tissue culture [J].

Igelmund, P ;

Fleischmann, BK ;

Fischer, IR ;

Soest, J ;

Gryshchenko, O ;

Böhm-Pinger, MM ;

Sauer, H ;

Liu, QH ;

Hescheler, J .

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1999, 437 (05) :669-679

[10] Generation of multipotent cell lines from a distinct population of male germ line stem cells [J].

Izadyar, Fariborz ;

Paul, Francis ;

Math, Jeol ;

Slepko, Natalia ;

Wang, Tracy ;

Gonzalez, Rafael ;

Ramos, Thomas ;

Howerton, Kyle ;

Sayre, Chauncey ;

Silva, Francisco .

REPRODUCTION, 2008, 135 (06) :771-784

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