A catalase microbiosensor for detecting hydrogen peroxide

被引：0

作者：

Emerson, D

Peteu, SF

Worden, RM

机构：

[1] MICHIGAN STATE UNIV,DEPT MICROBIOL,E LANSING,MI 48824

[2] MICHIGAN STATE UNIV,DEPT CHEM ENGN,E LANSING,MI 48824

来源：

BIOTECHNOLOGY TECHNIQUES | 1996年 / 10卷 / 09期

关键词：

D O I：

暂无

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A microbiosensor for hydrogen peroxide (H2O2) was constructed by immobilizing catalase in a polyacrylamide gel on the tip of a Clark-type oxygen microelectrode. The outer tip diameter was 15-40 mu m. The sensors had response times of 0.7-1.2 s, and could detect as little as 2-4 mu M H2O2. They could measure with a spatial resolution of about 100 mu m and remained operational for up to three weeks.

引用

页码：673 / 678

页数：6

共 6 条

[1]

AIZAWA M, 1974, ANAL CHIM ACTA, V69, P41

[2]

[Anonymous], IMMUNOLOGY INTRO

[3] AMPEROMETRIC SENSORS FOR PEROXIDE, CHOLINE, AND ACETYLCHOLINE BASED ON ELECTRON-TRANSFER BETWEEN HORSERADISH-PEROXIDASE AND A REDOX POLYMER [J].