A Dual-Color Quantum Dots Encoded Frit-Based Immunoassay for Visual Detection of Aflatoxin M1 and Pirlimycin Residues in Milk

被引:23
作者
Jiang, Wenxiao [1 ]
Beloglazova, Natalia V. [2 ]
Luo, Pengjie [3 ]
Guo, Ping [4 ]
Lin, Guimiao [1 ]
Wang, Xiaomei [1 ]
机构
[1] Shenzhen Univ, Sch Basic Med Sci, Dept Physiol, Hlth Sci Ctr, Shenzhen 518060, Peoples R China
[2] Univ Ghent, Lab Food Anal, Dept Bioanal, Harelbekestr 72, B-9000 Ghent, Belgium
[3] China Natl Ctr Food Safety Risk Assessment, Key Lab Food Safety Risk Assessment, Minist Hlth, Beijing 100021, Peoples R China
[4] JiangXi Entry Exit Inspect & Quarantine Bur, Technol Ctr, Nanchang 330038, Jiangxi, Peoples R China
基金
中国国家自然科学基金;
关键词
aflatoxin M-1; pirlimycin; immunoassay; milk; food safety; LINKED-IMMUNOSORBENT-ASSAY; EDIBLE ANIMAL-TISSUES; DIRECT HAPTEN LINKAGE; MONOCLONAL-ANTIBODY; MICROTITER PLATES; UHPLC-MS/MS; FLUORESCENCE; CLINDAMYCIN; SULFONAMIDE; ZEARALENONE;
D O I
10.1021/acs.jafc.6b05337
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Mycotoxins and antibacterial agents are the main chemical hazards that lead to several health problems. Nowadays, multiplex immunoassay is a primary goal throughout the world. Here, aflatoxin M-1, and pirlimycin were selected as models, and a novel dual colorimetric encoded frit-based immunoassay was developed for simultaneously screening of aflatoxin M-1, and pirlimycin residues in milk. This multiplex fit-based immunoassay combined two monoclonal antibodies to extend the spectrum of analytes and to enable detection of two classes of analytes in a single test. The cutoff values were 0.02 mu g/kg for aflatoxin M-1, and 0.5 mu g/kg for pirlimycin, which satisfied the requirement to measure the maximum residue levels. The novel colorimetric frit-based immunoassay has the advantage of high throughput, short analysis time, reduced overall cost per assay, and can be used as a rapid screening technique for simultaneously detecting aflatoxin M-1 and pirlimycin residues in milk.
引用
收藏
页码:1822 / 1828
页数:7
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