Fragile X-related protein and VIG associate with the RNA interference machinery

被引：485

作者：

Caudy, AA ^{[1
]}

Myers, M ^{[1
]}

Hannon, GJ ^{[1
]}

Hammond, SM ^{[1
]}

机构：

[1] Cold Spring Harbor Lab, Watson Sch Biol Sci, Cold Spring Harbor, NY 11724 USA

来源：

GENES & DEVELOPMENT | 2002年 / 16卷 / 19期

关键词：

dFXR; VIG; RNA interference; Ago-2; miRNA; Ago-1;

D O I：

10.1101/gad.1025202

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

RNA interference (RNAi) is a flexible gene silencing mechanism that responds to double-stranded RNA by suppressing homologous genes. Here, we report the characterization of RNAi effector complexes (RISCs) that contain small interfering RNAs and microRNAs (miRNAs). We identify two putative RNA-binding proteins, the Drosophila homolog of the fragile X mental retardation protein (FMRP), dFXR, and VIG (Vasa intronic gene), through their association with RISC. FMRP, the product of the human fragile X locus, regulates the expression of numerous mRNAs via an unknown mechanism. The possibility that dFXR, and potentially FMRP, use, at least in part, an RNAi-related mechanism for target recognition suggests a potentially important link between RNAi and human disease.

引用

页码：2491 / 2496

页数：6

共 43 条

[1] Advances in understanding of fragile X pathogenesis and FMRP function, and in identification of X linked mental retardation genes [J].

Bardoni, B ;

Mandel, JL .

CURRENT OPINION IN GENETICS & DEVELOPMENT, 2002, 12 (03) :284-293

[2] Role for a bidentate ribonuclease in the initiation step of RNA interference [J].

Bernstein, E ;

Caudy, AA ;

Hammond, SM ;

Hannon, GJ .

NATURE, 2001, 409 (6818) :363-366

[3]

Bernstein E, 2001, RNA, V7, P1509

[4] Microarray identification of FMRP-associated brain mRNAs and altered mRNA translational profiles in fragile X syndrome [J].

Brown, V ;

Jin, P ;

Ceman, S ;

Darnell, JC ;

O'Donnell, WT ;

Tenenbaum, SA ;

Jin, XK ;

Feng, Y ;

Wilkinson, KD ;

Keene, JD ;

Darnell, RB ;

Warren, ST .

CELL, 2001, 107 (04) :477-487

[5] Gemin4: A novel component of the SMN complex that is found in both gems and nucleoli [J].

Charroux, B ;

Pellizzoni, L ;

Perkinson, RA ;

Yong, J ;

Shevchenko, A ;

Mann, M ;

Dreyfuss, G .

JOURNAL OF CELL BIOLOGY, 2000, 148 (06) :1177-1186

[6] Fragile X mental retardation protein targets G quartet mRNAs important for neuronal function [J].

Darnell, JC ;

Jensen, KB ;

Jin, P ;

Brown, V ;

Warren, ST ;

Darnell, RB .

CELL, 2001, 107 (04) :489-499

[7] A POINT MUTATION IN THE FMR-1 GENE ASSOCIATED WITH FRAGILE-X MENTAL-RETARDATION [J].

DEBOULLE, K ;

VERKERK, AJMH ;

REYNIERS, E ;

VITS, L ;

HENDRICKX, J ;

VANROY, B ;

VANDENBOS, F ;

DEGRAAFF, E ;

OOSTRA, BA ;

WILLEMS, PJ .

NATURE GENETICS, 1993, 3 (01) :31-35

[8] Drosophila lacking dfmr1 activity show defects in circadian output and fail to maintain courtship interest [J].

Dockendorff, TC ;

Su, HS ;

McBride, SMJ ;

Yang, ZH ;

Choi, CH ;

Siwicki, KK ;

Sehgal, A ;

Jongens, TA .

NEURON, 2002, 34 (06) :973-984

[9] Using RNA interference to identify genes required for RNA interference [J].

Dudley, NR ;

Labbé, JC ;

Goldstein, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (07) :4191-4196

[10] The fragile X mental retardation protein is a ribonucleoprotein containing both nuclear localization and nuclear export signals [J].

Eberhart, DE ;

Malter, HE ;

Feng, Y ;

Warren, ST .

HUMAN MOLECULAR GENETICS, 1996, 5 (08) :1083-1091

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