Genetic analysis of foot-and-mouth disease virus serotype A of Indian origin and detection of positive selection and recombination in leader protease- and capsid-coding regions

被引:15
作者
Nagendrakumar, S. B. [1 ]
Madhanmohan, M. [1 ]
Rangarajan, P. N. [2 ]
Srinivasan, V. A. [1 ]
机构
[1] Indian Immunol Ltd, Ctr Res & Dev, Hyderabad 500032, Andhra Pradesh, India
[2] Indian Inst Sci, Dept Biochem, Bangalore 560080, Karnataka, India
关键词
Foot-and-mouth disease virus; phylogeny; Picornaviridae; positive selection; recombination; NEUTRALIZING ANTIGENIC SITES; PHYLOGENETIC ANALYSIS; SEQUENCE ALIGNMENT; A VIRUSES; IDENTIFICATION; VP1; STRAINS; VACCINE; DIFFERENTIATION; APHTHOVIRUS;
D O I
10.1007/s12038-009-0011-9
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The leader protease (L-pro) and capsid-coding sequences (P1) constitute approximately 3 kb of the foot-and-mouth disease virus (FMDV). We studied the phylogenetic relationship of 46 FMDV serotype A isolates of Indian origin collected during the period 1968-2005 and also eight vaccine strains using the neighbour-joining tree and Bayesian tree methods. The viruses were categorized under three major groups - Asian, Euro-South American and European. The Indian isolates formed a distinct genetic group among the Asian isolates. The Indian isolates were further classified into different genetic subgroups (<5% divergence). Post-1995 isolates were divided into two subgroups while a few isolates which originated in the year 2005 from Andhra Pradesh formed a separate group. These isolates were closely related to the isolates of the 1970s. The FMDV isolates seem to undergo reverse mutation or convergent evolution wherein sequences identical to the ancestors are present in the isolates in circulation. The eight vaccine strains included in the study were not related to each other and belonged to different genetic groups. Recombination was detected in the L-pro region in one isolate (A IND 20/82) and in the VP1 coding 1D region in another isolate (A RAJ 21/96). Positive selection was identified at aa positions 23 in the L-pro (P<0.05; 0.046*) and at aa 171 in the capsid protein VP1 (P<0.01; 0.003**).
引用
收藏
页码:85 / 101
页数:17
相关论文
共 51 条
  • [1] Antigenic sites of foot-and-mouth disease virus (FMDV): an analysis of the specificities of anti-FMDV antibodies after vaccination of naturally susceptible host species
    Aggarwal, N
    Barnett, PV
    [J]. JOURNAL OF GENERAL VIROLOGY, 2002, 83 : 775 - 782
  • [2] Extensive antigenic and genetic variation among foot-and-mouth disease type A viruses isolated from the 1994 and 1995 foci in Sao Paulo, Brazil
    Araujo, JP
    Montassier, HJ
    Pinto, AA
    [J]. VETERINARY MICROBIOLOGY, 2002, 84 (1-2) : 15 - 27
  • [3] A COMPARATIVE-STUDY OF SEROLOGICAL AND BIOCHEMICAL METHODS FOR STRAIN DIFFERENTIATION OF FOOT-AND-MOUTH-DISEASE TYPE-A VIRUSES
    ARMSTRONG, RM
    SAMUEL, AR
    CARPENTER, WC
    KANT, R
    KNOWLES, NJ
    [J]. VETERINARY MICROBIOLOGY, 1994, 39 (3-4) : 285 - 298
  • [4] VARIATION AMONG STRAINS OF TYPE-A FOOT-AND-MOUTH-DISEASE VIRUS IN EASTERN MEDITERRANEAN REGION 1964-1972
    ARROWSMITH, AEM
    [J]. JOURNAL OF HYGIENE, 1975, 75 (03) : 387 - 397
  • [5] AZAD HM, 1995, ACTA VIROL, V39, P193
  • [6] ANALYSIS OF NEUTRALIZING ANTIGENIC SITES ON THE SURFACE OF TYPE-A12 FOOT-AND-MOUTH-DISEASE VIRUS
    BAXT, B
    VAKHARIA, V
    MOORE, DM
    FRANKE, AJ
    MORGAN, DO
    [J]. JOURNAL OF VIROLOGY, 1989, 63 (05) : 2143 - 2151
  • [7] Belwal L M, 1987, Rev Sci Tech, V6, P115, DOI 10.20506/rst.6.1.283
  • [8] Belwal L M, 1986, Rev Sci Tech, V5, P723, DOI 10.20506/rst.5.3.264
  • [9] HOST-CELL SELECTION OF ANTIGENIC VARIANTS OF FOOT-AND-MOUTH-DISEASE VIRUS
    BOLWELL, C
    BROWN, AL
    BARNETT, PV
    CAMPBELL, RO
    CLARKE, BE
    PARRY, NR
    OULDRIDGE, EJ
    BROWN, F
    ROWLANDS, DJ
    [J]. JOURNAL OF GENERAL VIROLOGY, 1989, 70 : 45 - 57
  • [10] INTERTYPIC GENOMIC REARRANGEMENTS OF POLIOVIRUS STRAINS IN VACCINEES
    CAMMACK, N
    PHILLIPS, A
    DUNN, G
    PATEL, V
    MINOR, PD
    [J]. VIROLOGY, 1988, 167 (02) : 507 - 514