Influence of the antibiotic timentin on plant regeneration of tomato (Lycopersicon esculentum Mill.) cultivars

被引：55

作者：

Costa, MGC ^{[1
]}

Nogueira, FTS ^{[1
]}

Figueira, ML ^{[1
]}

Otoni, WC ^{[1
]}

Brommonschenkel, SH ^{[1
]}

Cecon, PR ^{[1
]}

机构：

[1] Univ Fed Vicosa, Dept Biol Vegetal & Nucl Biotecnol Aplicada & Agr, BR-36571000 Vicosa, MG, Brazil

来源：

PLANT CELL REPORTS | 2000年 / 19卷 / 03期

关键词：

Lycopersicon esculentum; antibiotics; ticarcillin/potassium clavulanate; rooting; morphogenesis;

D O I：

10.1007/s002990050021

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Cotyledon explants of tomato (Lycopersicon esculentum Mill. cvs 'Santa Clara', 'Firme' mutant, 'IPA-5' and 'IPA-6') were excised from 8- to 10-day-old in vitro-grown seedlings. Four different shoot induction media supplemented with timentin (300 mg l(-1)) were screened. When cotyledon explants were cultured on MS-based medium with 1.0 mg l(-1) zeatin plus 0.1 mg l(-1) IAA and supplemented with timentin, higher regeneration frequencies and a greater number of elongated shoots were obtained. It was observed that timentin caused an increase in the morphogenesis of in vitro cotyledon explants of tomato cultivars. In two of three cultivars tested, rooting of shoots was positively influenced, both in the presence and absence of timentin in the rooting medium, among shoots regenerated from explants derived from timentin-supplemented medium. The results confirm those of a previous investigation on the beneficial effects of this class of antibiotics on tomato regeneration and, consequently, its reliability for use in the transformation of this species.

引用

页码：327 / 332

页数：6

共 25 条

[21] GROWTH-PROMOTING ACTIVITY OF CERTAIN PENICILLINS ON CULTIVATED CELLS OF BOUVARDIA-TERNIFOLIA [J].

ROBERT, ML ;

FLORES, MR ;

LOYOLAVARGAS, VM .

PHYTOCHEMISTRY, 1989, 28 (10) :2659-2662

[22] TRANSFORMATION AND REGENERATION OF 2 CULTIVARS OF PEA (PISUM-SATIVUM L) [J].

SCHROEDER, HE ;

SCHOTZ, AH ;

WARDLEYRICHARDSON, T ;

SPENCER, D ;

HIGGINS, TJV .

PLANT PHYSIOLOGY, 1993, 101 (03) :751-757

[23]

Teng WL, 1997, PLANT CELL REP, V16, P531, DOI [10.1007/s002990050273, 10.1007/BF01142318]

[24] FACTORS INFLUENCING TRANSFORMATION FREQUENCY OF TOMATO (LYCOPERSICON-ESCULENTUM) [J].

VANROEKEL, JSC ;

DAMM, B ;

MELCHERS, LS ;

HOEKEMA, A .

PLANT CELL REPORTS, 1993, 12 (11) :644-647

[25]

ZIMMERMAN TW, 1995, IN VITRO CELL DEV, V31, pA70

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