Polyethyleneimine-grafted poly (hydroxyethyl methacrylate-co-glycidyl methacrylate) membranes for reversible glucose oxidase immobilization

被引：62
|
作者：
Arica, MY [1 ]
Bayramoglu, G [1 ]
机构：
[1] Kirikkale Univ, Fac Sci, Biochem Proc & Biomat Res Lab, TR-71450 Kirikkale, Turkey
来源：
BIOCHEMICAL ENGINEERING JOURNAL | 2004年 / 20卷 / 01期
关键词：
membranes; immobilization; adsorption; enzyme technology; enzyme; glucose oxidase;
D O I：
10.1016/j.bej.2004.04.008
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Glucose oxidase (GOD) was immobilized onto polyethyleneimide (PEI) grafted poly(hydroxyethyl methacrylate-co-glycidyl methacrylate) (poly(HEMA-co-GMA)) membranes. The maximum GOD immobilization capacities of the poly(HEMA-co-GMA-4)-PEI membrane were 84 mug/cm(2). The activities of GOD-immobilized onto poly(HEMA-co-GMA-1-5)-PEI membranes were in the range 7.6-14.5 U/cm(2). The storage conditions for the immobilized enzyme preparations were studied and the immobilized enzymes had a long-storage stability (only 7 and 24% activity decrease in 3 months under wet and dry storage conditions, respectively). (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码：73 / 77
页数：5
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