Polyethyleneimine-grafted poly (hydroxyethyl methacrylate-co-glycidyl methacrylate) membranes for reversible glucose oxidase immobilization

被引：62
|
作者：
Arica, MY [1 ]
Bayramoglu, G [1 ]
机构：
[1] Kirikkale Univ, Fac Sci, Biochem Proc & Biomat Res Lab, TR-71450 Kirikkale, Turkey
来源：
BIOCHEMICAL ENGINEERING JOURNAL | 2004年 / 20卷 / 01期
关键词：
membranes; immobilization; adsorption; enzyme technology; enzyme; glucose oxidase;
D O I：
10.1016/j.bej.2004.04.008
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Glucose oxidase (GOD) was immobilized onto polyethyleneimide (PEI) grafted poly(hydroxyethyl methacrylate-co-glycidyl methacrylate) (poly(HEMA-co-GMA)) membranes. The maximum GOD immobilization capacities of the poly(HEMA-co-GMA-4)-PEI membrane were 84 mug/cm(2). The activities of GOD-immobilized onto poly(HEMA-co-GMA-1-5)-PEI membranes were in the range 7.6-14.5 U/cm(2). The storage conditions for the immobilized enzyme preparations were studied and the immobilized enzymes had a long-storage stability (only 7 and 24% activity decrease in 3 months under wet and dry storage conditions, respectively). (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码：73 / 77
页数：5
相关论文
共 50 条
  • [1] Chitosan-grafted poly(hydroxyethyl methacrylate-co-glycidyl methacrylate) membranes for reversible enzyme immobilization
    Arica, M. Yakup
    Yilmaz, Meltem
    Bayramoglu, Gulay
    JOURNAL OF APPLIED POLYMER SCIENCE, 2007, 103 (05) : 3084 - 3093
  • [2] Poly(hydroxyethyl methacrylate-co-glycidyl methacrylate) reactive membrane utilised for cholesterol oxidase immobilisation
    Akgöl, S
    Bayramoglu, G
    Kacar, Y
    Denizli, A
    Arica, MY
    POLYMER INTERNATIONAL, 2002, 51 (12) : 1316 - 1322
  • [3] Preparation of Novel Poly(hydroxyethyl methacrylate-co-glycidyl methacrylate)-Grafted Core-Shell Magnetic Chitosan Microspheres and Immobilization of Lactase
    Zhao, Wei
    Yang, Rui-Jin
    Qian, Ting-Ting
    Hua, Xiao
    Zhang, Wen-Bin
    Katiyo, Wendy
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2013, 14 (06) : 12073 - 12089
  • [4] Preparation and Properties of Cryogel Based on Poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate)
    Wan, Binbin
    Li, Junying
    Ma, Feng
    Yu, Ning
    Zhang, Wenjing
    Jiang, Lujie
    Wei, Hengshan
    LANGMUIR, 2019, 35 (09) : 3284 - 3294
  • [5] IMMOBILIZATION OF GLUCOSE-OXIDASE IN POLY(2-HYDROXYETHYL METHACRYLATE) MEMBRANES
    ARICA, Y
    HASIRCI, VN
    BIOMATERIALS, 1987, 8 (06) : 489 - 495
  • [6] Poly(urethane methacrylate-co-glycidyl methacrylate)-supported-polypropylene biphasic membrane for lipase immobilization
    Pujari, N. S.
    Vaidya, Bx
    Bagalkote, S.
    Ponrathnam, S.
    Nene, Sanjay
    JOURNAL OF MEMBRANE SCIENCE, 2006, 285 (1-2) : 395 - 403
  • [7] Catalytic activity of bovine lactoperoxidase supported on macroporous poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate)
    Di Nino, G
    Turacchio, M
    D'Archivio, AA
    Lora, S
    Corain, B
    Antonini, G
    REACTIVE & FUNCTIONAL POLYMERS, 2004, 61 (03): : 411 - 419
  • [8] Bioactive interpenetrating hybrids of poly(hydroxyethyl methacrylate-co-glycidyl methacrylate): Effect of polysaccharide types on structural peculiarities and multifunctionality
    Er, Mertcan
    Orakdogen, Nermin
    INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2024, 254
  • [9] Preparation and application of spacer-arm-attached poly (hydroxyethyl methacrylate-co-glycidyl methacrylate) films for urease immobilisation
    Bayramoglu, G
    Altinok, H
    Bulut, A
    Denizli, A
    Arica, MY
    REACTIVE & FUNCTIONAL POLYMERS, 2003, 56 (02): : 111 - 121
  • [10] Magnetic Hydrophilic Poly(2-Hydroxyethyl Methacrylate-co-Glycidyl Methacrylate) Microspheres for DNA Isolation from Faeces
    Trachtova, Stepanka
    Obermajer, Tanja
    Spanova, Alena
    Matijasic, Bojana Bogovic
    Rogelj, Irena
    Horak, Daniel
    Rittich, Bohuslav
    MOLECULAR CRYSTALS AND LIQUID CRYSTALS, 2012, 555 : 263 - 270
12345