Polyethyleneimine-grafted poly (hydroxyethyl methacrylate-co-glycidyl methacrylate) membranes for reversible glucose oxidase immobilization

被引：62
作者：
Arica, MY [1 ]
Bayramoglu, G [1 ]
机构：
[1] Kirikkale Univ, Fac Sci, Biochem Proc & Biomat Res Lab, TR-71450 Kirikkale, Turkey
来源：
BIOCHEMICAL ENGINEERING JOURNAL | 2004年 / 20卷 / 01期
关键词：
membranes; immobilization; adsorption; enzyme technology; enzyme; glucose oxidase;
D O I：
10.1016/j.bej.2004.04.008
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Glucose oxidase (GOD) was immobilized onto polyethyleneimide (PEI) grafted poly(hydroxyethyl methacrylate-co-glycidyl methacrylate) (poly(HEMA-co-GMA)) membranes. The maximum GOD immobilization capacities of the poly(HEMA-co-GMA-4)-PEI membrane were 84 mug/cm(2). The activities of GOD-immobilized onto poly(HEMA-co-GMA-1-5)-PEI membranes were in the range 7.6-14.5 U/cm(2). The storage conditions for the immobilized enzyme preparations were studied and the immobilized enzymes had a long-storage stability (only 7 and 24% activity decrease in 3 months under wet and dry storage conditions, respectively). (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码：73 / 77
页数：5
相关论文
共 16 条
  • [1] Reversible immobilization of urease onto Procion Brown MX-5BR-Ni(II) attached polyamide hollow-fibre membranes
    Akgöl, S
    Yalçinkaya, Y
    Bayramoglu, G
    Denizli, A
    Arica, MY
    [J]. PROCESS BIOCHEMISTRY, 2002, 38 (05) : 675 - 683
  • [2] [Anonymous], QUANTITATIVE ORGANIC
  • [3] Covalent immobilisation of invertase onto a reactive film composed of 2-hydroxyethyl methacrylate and glycidyl methacrylate:: properties and application in a continuous flow system
    Bayramoglu, G
    Akgöl, S
    Bulut, A
    Denizli, A
    Arica, MY
    [J]. BIOCHEMICAL ENGINEERING JOURNAL, 2003, 14 (02) : 117 - 126
  • [4] BAYRAMOGLU G, 2002, COLLOID SURF A, P41
  • [5] Immobilization of lipase on a polymeric microsphere with an epoxy group prepared by radiation-induced polymerization
    Choi, SH
    Lee, KP
    Kang, HD
    [J]. JOURNAL OF APPLIED POLYMER SCIENCE, 2003, 88 (05) : 1153 - 1161
  • [6] Applications of laccases and tyrosinases (phenoloxidases) immobilized on different supports:: a review
    Durán, N
    Rosa, MA
    D'Annibale, A
    Gianfreda, L
    [J]. ENZYME AND MICROBIAL TECHNOLOGY, 2002, 31 (07) : 907 - 931
  • [7] Immobilization of polyphenol oxidase on chitosan-coated polysulphone capillary membranes for improved phenolic effluent bioremediation
    Edwards, W
    Leukes, WD
    Rose, PD
    Burton, SG
    [J]. ENZYME AND MICROBIAL TECHNOLOGY, 1999, 25 (8-9) : 769 - 773
  • [8] Enzymatic membrane reactors for biodegradation of recalcitrant compounds.: Application to dye decolourisation
    López, C
    Mielgo, I
    Moreira, MT
    Feijoo, G
    Lema, JM
    [J]. JOURNAL OF BIOTECHNOLOGY, 2002, 99 (03) : 249 - 257
  • [9] Mateo C, 2000, BIOTECHNOL BIOENG, V68, P98, DOI 10.1002/(SICI)1097-0290(20000405)68:1<98::AID-BIT12>3.0.CO
  • [10] 2-T
12