An altered intestinal mucosal microbiome in HIV-1 infection is associated with mucosal and systemic immune activation and endotoxemia

被引：397

作者：

Dillon, S. M. ^{[1
]}

Lee, E. J. ^{[1
]}

Kotter, C. V. ^{[1
]}

Austin, G. L. ^{[1
]}

Dong, Z. ^{[1
]}

Hecht, D. K. ^{[1
]}

Gianella, S. ^{[2
]}

Siewe, B. ^{[3
]}

Smith, D. M. ^{[2
]}

Landay, A. L. ^{[3
]}

Robertson, C. E. ^{[4
]}

Frank, D. N. ^{[1
,5
]}

Wilson, C. C. ^{[1
]}

机构：

[1] Univ Colorado, Dept Med, Aurora, CO 80045 USA

[2] Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA

[3] Rush Univ, Med Ctr, Dept Immunol & Microbiol, Chicago, IL 60612 USA

[4] Univ Colorado, Dept Mol Cellular & Dev Biol, Boulder, CO 80309 USA

[5] Univ Colorado, Microbiome Res Consortium, Aurora, CO USA

来源：

MUCOSAL IMMUNOLOGY | 2014年 / 7卷 / 04期

基金：

美国国家卫生研究院;

关键词：

T-CELL DEPLETION; DENDRITIC CELLS; TRANSLOCATION; METABOLISM; BARRIER; SOFTWARE; BUTYRATE; BACTERIA; PRIMERS; DRIVEN;

D O I：

10.1038/mi.2013.116

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Human immunodeficiency virus-1 (HIV-1) infection disrupts the intestinal immune system, leading to microbial translocation and systemic immune activation. We investigated the impact of HIV-1 infection on the intestinal microbiome and its association with mucosal T-cell and dendritic cell (DC) frequency and activation, as well as with levels of systemic T-cell activation, inflammation, and microbial translocation. Bacterial 16S ribosomal DNA sequencing was performed on colon biopsies and fecal samples from subjects with chronic, untreated HIV-1 infection and uninfected control subjects. Colon biopsies of HIV-1-infected subjects had increased abundances of Proteobacteria and decreased abundances of Firmicutes compared with uninfected donors. Furthermore at the genus level, a significant increase in Prevotella and decrease in Bacteroides was observed in HIV-1-infected subjects, indicating a disruption in the Bacteroidetes bacterial community structure. This HIV-1-associated increase in Prevotella abundance was associated with increased numbers of activated colonic Tcells and myeloid DCs. Principal coordinates analysis demonstrated an HIV-1-related change in the microbiome that was associated with increased mucosal cellular immune activation, microbial translocation, and blood T-cell activation. These observations suggest that an important relationship exists between altered mucosal bacterial communities and intestinal inflammation during chronic HIV-1 infection.

引用

页码：983 / 994

页数：12

共 48 条

[21] Plasma Levels of Bacterial DNA Correlate with Immune Activation and the Magnitude of Immune Restoration in Persons with Antiretroviral-Treated HIV Infection [J].