Isolation and characterization of 13 microsatellite loci in Rhinolophus pusillus (least horseshoe bat) with cross-amplification in five related species

被引：4
作者：
Hua, Panyu [1 ]
Guo, Tingting [1 ]
Liu, Wenchao [1 ]
Zhang, Shuyi [1 ]
Rossiter, Stephen J. [2 ]
机构：
[1] E China Normal Univ, Sch Life Sci, Shanghai 200062, Peoples R China
[2] Univ London, Sch Biol & Chem Sci, London E1 4NS, England
来源：
CONSERVATION GENETICS | 2009年 / 10卷 / 03期
关键词：
Microsatellite loci; Rhinolophus pusillus; Genetic structure;
D O I：
10.1007/s10592-008-9586-1
中图分类号：
X176 [生物多样性保护];
学科分类号：
090705 ;
摘要：
We used the enriched genomic library method to isolate and characterize dinucleotide microsatellite loci in the least horseshoe bat, Rhinolophus pusillus. Seventeen loci were obtained and tested on 31 individuals sampled from Guangxi Province in southern China. Thirteen of these markers were polymorphic with expected heterozygosity ranging from 0.821 to 0.909. A total of 164 alleles were detected and the number of alleles per locus ranged from 9 to 16 (mean 12.6). These polymorphic markers will be used to assess population structure in R. pusillus. In addition, successful cross-amplification in five congeneric bat species suggests most of these markers will also be useful for studying related species.
引用
收藏
页码：597 / 600
页数：4
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