Control of microtubule dynamics by the antagonistic activities of XMAP215 and XKCM1 in Xenopus egg extracts

被引：240

作者：

Tournebize, R

Popov, A

Kinoshita, K

Ashford, AJ

Rybina, S

Pozniakovsky, A

Mayer, TU

Walczak, CE

Karsenti, E

Hyman, AA

机构：

[1] European Mol Biol Lab, Cell Biol Program, D-69117 Heidelberg, Germany

[2] Indiana Univ, Med Sci Program, Bloomington, IN 47405 USA

来源：

NATURE CELL BIOLOGY | 2000年 / 2卷 / 01期

关键词：

D O I：

10.1038/71330

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Microtubules are dynamic polymers that move stochastically between periods of growth and shrinkage, a property known as dynamic instability, Here, to investigate the mechanisms regulating microtubule dynamics in Xenopus egg extracts, we have cloned the complementary DNA encoding the microtubule-associated protein XMAP215 and investigated the function of the XMAP215 protein. Immunodepletion of XMAP215 indicated that it is a major microtubule-stabilizing factor in Xenopus egg extracts. During interphase, XMAP215 stabilizes microtubules primarily by opposing the activity of the destabilizing factor XKCM1, a member of the kinesin superfamily. These results indicate that microtubule dynamics in Xenopus egg extracts are regulated by a balance between a stabilizing factor, XMAP215, and a destabilizing factor, XKCM1.

引用

页码：13 / 19

页数：7