Acidic extracellular proteases from microrganisms of fairly acidic niche

被引:10
作者
Bossi, Alessandra [1 ]
Bonizzato, Luca [1 ]
Zapparoli, Giacomo [1 ]
机构
[1] Univ Verona, Dept Sci & Technol, I-37134 Verona, Italy
关键词
Lactobacillus hilgardii; Acetobacter aceti; Brettanomyces bruxellensis; extracellular protease; two-dimensional electrophoresis; zymogram;
D O I
10.2174/092986606777790647
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The protein population secreted from three wine-contaminant microorganisms was studied by two-dimensional electrophoresis and screened for proteases. Proteolytic enzymes were electrophoretically purified and their activity, optimum pH and temperature determined. The protease of Acetobacter aceti maintained its activity over the range of pHs 3.0-5.0, thus being of potential biotechnological interest.
引用
收藏
页码:737 / 741
页数:5
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