Transforming growth factor-β1 induces connective tissue growth factor expression and promotes peritoneal metastasis of gastric cancer

被引：12
作者：
Lv, Ling [1 ]
Liu, Fu-Rong [2 ]
Na, Di [3 ]
Xu, Hui-Mian [3 ]
Wang, Zhen-Ning [3 ]
Jiang, Cheng-Gang [3 ]
机构：
[1] China Med Univ, Dept Thorac Surg, Affiliated Hosp 1, Shenyang, Liaoning, Peoples R China
[2] China Med Univ, Dept Cell Biol, Shenyang, Liaoning, Peoples R China
[3] China Med Univ, Dept Surg Oncol & Gen Surg, Key Lab Precis Diag & Treatment Gastrointestinal, Affiliated Hosp 1,Minist Educ, Shenyang, Liaoning, Peoples R China
来源：
BIOSCIENCE REPORTS | 2020年 / 40卷
关键词：
TGF-BETA; SMAD; ACTIVATION; SURVIVAL; CELLS;
D O I：
10.1042/BSR20201501
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Transforming growth factor-beta(1) (TGF-beta(1)) is involved in human cancer development and progression. Nonetheless, the role of TGF-beta(1) as regards peritoneal metastasis of gastric cancer has not been completely characterized. In the present study, we investigated the exact role of TGF-beta(1) on peritoneal metastasis of gastric cancer. The results indicated that human peritoneal mesothelial cells (HPMCs) exposed to TGF-beta(1) or serum-free conditional medium (SF-CM) of SGC7901 that produced a large amount of TGF-beta(1) became exfoliated, apoptosis and exhibited signs of injury, and the tumor-mesothelial cell adhesion significantly increased. Connective tissue growth factor (CTGF) expression was also increased when HPMCs were exposed to TGF-beta(1) or SF-CM of SGC7901. However, these effects were significantly decreased when HPMCs were exposed to SF-CM of SGC7901-TGF beta S, a TGF-beta(1) knockdown stable cell line. Animal studies revealed that nude mice injected with SGC7901-TGF beta S cells featured a smaller number of peritoneal seeding nodules and lower expression of CTGF in ascites than the control cell lines. These findings suggest that TGF-beta(1) promotes peritoneal metastasis of gastric cancer and induces CTGF expression. Therefore, blockage of TGF-beta(1) or TGF-beta(1) signaling pathway might prevent and treat peritoneal metastasis of gastric cancer.
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页数：14
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