PANEL REACTIVE ANTIBODY IN THALASSEMIC SERUM INHIBITS PROLIFERATION AND DIFFERENTIATION OF CORD BLOOD CD34+CELLS IN VITRO

被引：6
作者：
Fang, Jian-Pei [1 ]
Xu, Lu-Hong [1 ]
Yang, Xing-Ge [1 ]
Wu, Yan-Feng [1 ]
Weng, Wen-Jun [1 ]
Xu, Hong-Gui [1 ]
机构：
[1] Sun Yat Sen Univ, Affiliated Hosp 2, Dept Pediat, Guangzhou 510275, Guangdong, Peoples R China
来源：
PEDIATRIC HEMATOLOGY AND ONCOLOGY | 2009年 / 26卷 / 05期
基金：
中国国家自然科学基金;
关键词：
CD34+cells; colony-forming units; cord blood; panel reactive antibody; thalassemia; MARROW GRAFT-REJECTION; T-CELLS; TRANSPLANTATION; ENGRAFTMENT; RECIPIENTS; BARRIER; ASSAYS;
D O I：
10.1080/08880010902976098
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Thalassemic children are at a high risk of graft rejection in cord blood transplantation. To investigate this possible mechanism, the authors evaluated the effect of panel reactive antibody on the growth of CD34+ cells in vitro. On semisolid medium, CD34+ cells derived from cord blood were incubated with thalassemic serum, and colony-forming units were counted after 10 days of culture. After incubation with serum-specific panel reactive antibody, profound decreases were found in the numbers of CFU-GM, CFU-GEMM and BFU-E compared with controls. The results indicated that serum-specific panel reactive antibody might have an apparent inhibition effect on proliferation and differentiation of cord blood CD34+ cells.
引用
收藏
页码：338 / 344
页数：7
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