Strontium-incorporated mineralized PLLA nanofibrous membranes for promoting bone defect repair

被引：39

作者：

Han, Xiuguo ^{[1
]}

Zhou, Xiaojun ^{[1
]}

Qiu, Kexin ^{[2
]}

Feng, Wei ^{[2
]}

Mo, Huimin ^{[3
]}

Wang, Minqi ^{[1
]}

Wang, Jinwu ^{[1
]}

He, Chuanglong ^{[2
]}

机构：

[1] Shanghai Jiao Tong Univ, Shanghai Key Lab Orthopaed Implants, Dept Orthopaed Surg, Sch Med,Shanghai Peoples Hosp 9, Shanghai 200011, Peoples R China

[2] Donghua Univ, Coll Chem Chem Engn & Biotechnol, Shanghai 201620, Peoples R China

[3] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Sch Med, Dept Hematol, Shanghai 200011, Peoples R China

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2019年 / 179卷

基金：

中国国家自然科学基金; 中国博士后科学基金;

关键词：

Nanofibrous membrane; Electrodeposition; Strontium (Sr); Osteogenic differentiation; Bone regeneration; MESENCHYMAL STEM-CELLS; IN-VITRO; OSTEOGENIC DIFFERENTIATION; HYDROXYAPATITE COATINGS; MECHANICAL-PROPERTIES; COMPOSITE NANOFIBERS; RAPID MINERALIZATION; SCAFFOLDS; MATRIX; DRUG;

D O I：

10.1016/j.colsurfb.2019.04.011

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Mineralized scaffolds, which are fabricated by electrodeposition, mimic the chemistry of natural bone and have attracted a great amount of attention due to their rapid and simple production. In this study, mineralized electrospun polylactic acid (PLLA) nanofibrous membranes containing different amounts of strontium (Sr) were fabricated by an electrodeposition method for potential use in bone regeneration applications. In vitro assays, including cell proliferation and osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells (BMSCs) grown on these membranes and in vivo cranial bone defect repair assays, were carried out. It was found that mineral crystals could be uniformly deposited onto the electrospun nanofibrous membranes, while the morphologies of the formed crystals were affected by the amount of Sr. By analysis of the X-ray diffraction (XRD) measurements, the formed crystalline phase was dramatically affected by the incorporation of Sr, which drove a conversion from the hydroxyapatite (HA) phase to the dicalcium phosphate dehydrate (DCPD) phase. The release of Sr2+ from the Sr/PLLA nanofibrous membranes was monitored over 20 d, and the release rates of Ca2+ and PO43- from the Sr-incorporated samples were higher compared with those of the mineralized sample without Sr. In vitro cell proliferation experiments demonstrated that mineralized Sr/PLLA nanofibrous membranes could facilitate BMSC proliferation. Furthermore, the mineralized Sr/PLLA nanofibrous membranes induced a higher degree of osteogenic differentiation in the BMSCs compared with those of pure PLLA and mineralized PLLA, as determined by the results of alkaline phosphatase (ALP) activity, alizarin red (AR) and osteocalcin (OCN) staining and the expression of osteogenesis-related genes. More importantly, in vivo cranial defect experiments revealed that mineralized Sr/PLLA nanofibrous membranes promoted bone regeneration. These findings indicated that mineralized Sr/PLLA nanofibrous membranes are a promising material for bone tissue engineering.

引用

页码：363 / 373

页数：11

共 53 条

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