Glucose Biosensor Based on Nanocomposite Films of CdTe Quantum Dots and Glucose Oxidase

被引：159
作者：
Li, Xinyu [1 ]
Zhou, Yunlong [2 ]
Zheng, Zhaozhu [1 ]
Yue, Xiuli [3 ]
Dai, Zhifei [1 ]
Liu, Shaoqin [1 ,4 ]
Tang, Zhiyong [2 ]
机构：
[1] Harbin Inst Technol, Bio X Ctr, Nanomed & Biosensor Lab, Harbin 150080, Peoples R China
[2] Natl Ctr Nanosci & Technol, Beijing 100190, Peoples R China
[3] Harbin Inst Technol, Sch Municipal & Environm Engn, Harbin 150001, Peoples R China
[4] Harbin Inst Technol, Ctr Micro & Nanotechnol, Harbin 150080, Peoples R China
来源：
LANGMUIR | 2009年 / 25卷 / 11期
关键词：
CYTOCHROME-C; POLYELECTROLYTE; CDSE; NANOSTRUCTURES; NANOPARTICLES; PERMEABILITY; NANOCRYSTALS; OXIDATION; CHITOSAN; SYSTEMS;
D O I：
10.1021/la900066z
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A blood glucose sensor has been developed based on the multilayer films of CdTe semiconductor quantum dots (QDs) and glucose oxidase (GOD) by using the layer-by-layer assembly technique. When the composite films were contacted with glucose solution, the photoluminescence of QDs in the films was quickly quenched because the enzyme-catalyzed reaction product (H2O2) of GOD and glucose gave rise to the formation of surface defects on QDs. The quenching rate was a function of the concentration of glucose. The linear range and sensitivity for glucose determination could be adjusted by controlling the layers of QDs and GOD. The biosensor was used to successfully deter-mine the concentration of blood glucose in real serum samples without sample pretreatment and exhibited satisfactory reproducibility and accuracy.
引用
收藏
页码：6580 / 6586
页数：7
相关论文
共 61 条
  • [1] Semiconductor clusters, nanocrystals, and quantum dots
    Alivisatos, AP
    [J]. SCIENCE, 1996, 271 (5251) : 933 - 937
  • [2] Biomolecule-nanoparticle hybrids as functional units for nanobiotechnology
    Baron, Ronan
    Willner, Bilha
    Willner, Itamar
    [J]. CHEMICAL COMMUNICATIONS, 2007, (04) : 323 - 332
  • [3] QUANTUM CRYSTALLITES AND NONLINEAR OPTICS
    BRUS, L
    [J]. APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING, 1991, 53 (06): : 465 - 474
  • [4] Electron shuttling across the interface of CdSe nanoparticles monitored by femtosecond laser spectroscopy
    Burda, C
    Green, TC
    Link, S
    El-Sayed, MA
    [J]. JOURNAL OF PHYSICAL CHEMISTRY B, 1999, 103 (11): : 1783 - 1788
  • [5] Luminescent quantum dots for multiplexed biological detection and imaging
    Chan, WCW
    Maxwell, DJ
    Gao, XH
    Bailey, RE
    Han, MY
    Nie, SM
    [J]. CURRENT OPINION IN BIOTECHNOLOGY, 2002, 13 (01) : 40 - 46
  • [6] ELECTRODE SYSTEMS FOR CONTINUOUS MONITORING IN CARDIOVASCULAR SURGERY
    CLARK, LC
    LYONS, C
    [J]. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1962, 102 (01) : 29 - &
  • [7] Layer-by-layer biosensor assembly incorporating functionalized quantum dots
    Constantine, CA
    Gattás-Asfura, KM
    Mello, SV
    Crespo, G
    Rastogi, V
    Cheng, TC
    DeFrank, JJ
    Leblanc, RM
    [J]. LANGMUIR, 2003, 19 (23) : 9863 - 9867
  • [8] CORNISHBOWDEN A, 1979, FUNDAMENTALS ENZYME, P17
  • [9] The use of luminescent quantum dots for optical sensing
    Costa-Fernández, JM
    Pereiro, R
    Sanz-Medel, A
    [J]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2006, 25 (03) : 207 - 218
  • [10] Controlling the permeability of multilayered polyelectrolyte films through derivatization, cross-linking, and hydrolysis
    Dai, JH
    Jensen, AW
    Mohanty, DK
    Erndt, J
    Bruening, ML
    [J]. LANGMUIR, 2001, 17 (03) : 931 - 937
1234567