Colorimetric and fluorescence recognition of tryptophan and histidine using phthalaldehyde based probe: experimental, computational, cell imaging and fish tissue analysis

被引:7
作者
Ghosh, Abhijit [1 ]
Talukdar, Sandipan [2 ]
Ghosh, Koushik [2 ]
Das, Tanmoy [1 ]
Das, Debasis [1 ]
机构
[1] Univ Burdwan, Dept Chem, Burdwan 713104, W Bengal, India
[2] Univ Burdwan, Dept Zool, Aquaculture Lab, Burdwan 713104, W Bengal, India
关键词
ACID; BIOSENSOR; THERAPY; LEVEL;
D O I
10.1039/c4ra10158h
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A phthalaldehyde based colorimetric and fluorescence probe (BENPH), selective for tryptophan (Trp) and histidine (His) has been developed. BENPH and its Trp and His adducts are characterized by FTIR, H-1 NMR and ESI-Mass spectroscopy. Computational studies support the experimental findings. BENPH can image intracellular Trp and His under a fluorescence microscope. The developed method is used to determine Trp and His in fish muscle.
引用
收藏
页码:55286 / 55289
页数:4
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